{"id":2317,"date":"2022-08-25T12:12:20","date_gmt":"2022-08-25T03:12:20","guid":{"rendered":"https:\/\/www.chem.s.u-tokyo.ac.jp\/campbell\/?page_id=2317"},"modified":"2024-07-28T03:22:56","modified_gmt":"2024-07-28T12:22:56","slug":"projects_ja","status":"publish","type":"page","link":"https:\/\/campbell.chem.s.u-tokyo.ac.jp\/?page_id=2317","title":{"rendered":"Research (JA)"},"content":{"rendered":"<p>[et_pb_section bb_built=&#8221;1&#8243; fullwidth=&#8221;off&#8221; specialty=&#8221;off&#8221; next_background_color=&#8221;#000000&#8243;][et_pb_row][et_pb_column type=&#8221;4_4&#8243;][et_pb_text admin_label=&#8221;Language selector&#8221; _builder_version=&#8221;3.12.2&#8243;]<\/p>\n<p>&nbsp;<\/p>\n<p style=\"text-align: right;\"><span style=\"font-size: 18pt;\"><span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/www.chem.s.u-tokyo.ac.jp\/campbell\/projects\/\"><span style=\"color: #3366ff;\">English<\/span><\/a><\/span> \/ \u65e5\u672c\u8a9e<\/span><\/p>\n<p>&nbsp;<\/p>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row][et_pb_column type=&#8221;4_4&#8243;][et_pb_text admin_label=&#8221;Research outline JP&#8221; _builder_version=&#8221;3.12.2&#8243;]<\/p>\n<h1 style=\"text-align: center;\"><span style=\"font-size: 18pt; color: #000000;\"><strong>\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u5b50\u30c4\u30fc\u30eb\u306e\u5275\u6210\u3068\u6307\u5411\u6027\u9032\u5316<\/strong><\/span><\/h1>\n<p><span style=\"color: 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style=\"color: #333333;\">\u30ad\u30e3\u30f3\u30d9\u30eb\u7814\u7a76\u5ba4\u306e\u6700\u5148\u7aef\u7814\u7a76\u3067\u306f\u3001\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u3001\u7279\u5b9a\u306e\u751f\u5316\u5b66\u7684\u5909\u5316\u306b\u53cd\u5fdc\u3057\u3066\u86cd\u5149\u5f37\u5ea6\u3084\u8272\u304c\u5909\u5316\u3059\u308b\u52d5\u7684\u306a\u6307\u793a\u85ac\uff08\u30d0\u30a4\u30aa\u30bb\u30f3\u30b5\u30fc\uff09\u3078\u3068\u5909\u63db\u3057\u3066\u3044\u307e\u3059\u3002\u7279\u306b\u3001\u5229\u7528\u53ef\u80fd\u306a\u86cd\u5149\u8272\u306e\u7bc4\u56f2\u3092\u5e83\u3052\u3001\u3088\u308a\u8d64\u306b\u8fd1\u3044\u6ce2\u9577\u3078\u3068\u5909\u5316\u3055\u305b\u308b\u3053\u3068\u306b\u91cd\u70b9\u3092\u7f6e\u3044\u3066\u3044\u307e\u3059\u3002\u751f\u4f53\u7d44\u7e54\u306f\u8fd1\u8d64\u5916\u6ce2\u9577\u57df\u3067\u6700\u3082\u900f\u660e\u5ea6\u304c\u9ad8\u3044\u305f\u3081\u3001\u8d64\u8272\u5074\u306e\u6307\u793a\u85ac\u3092\u4f7f\u3048\u3070\u7d44\u7e54\u306e\u5965\u6df1\u304f\u307e\u3067\u53ef\u8996\u5316\u3067\u304d\u308b\u304b\u3089\u3067\u3059\u3002<\/span><\/p>\n<p><span style=\"color: #333333;\">\u4ee5\u4e0b\u306f\u3001\u3053\u308c\u307e\u3067\u306e\u6210\u679c\u3068\u73fe\u5728\u9032\u884c\u4e2d\u306e\u65b9\u5411\u6027\u306e\u30cf\u30a4\u30e9\u30a4\u30c8\u3067\u3059\u3002<\/span><\/p>\n<p>[\/et_pb_text][et_pb_image admin_label=&#8221;GFP image&#8221; _builder_version=&#8221;3.12.2&#8243; src=&#8221;https:\/\/www.chem.s.u-tokyo.ac.jp\/campbell\/wp-content\/uploads\/sites\/7\/2021\/02\/GFP-for-project-top.png&#8221; align=&#8221;center&#8221; max_width=&#8221;33%&#8221; animation_style=&#8221;fade&#8221; \/][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section bb_built=&#8221;1&#8243; background_color=&#8221;#ffffff&#8221; inner_shadow=&#8221;off&#8221; parallax=&#8221;off&#8221; parallax_method=&#8221;css&#8221; prev_background_color=&#8221;#000000&#8243; _builder_version=&#8221;3.12.2&#8243; next_background_color=&#8221;#ededed&#8221;][et_pb_row _builder_version=&#8221;3.12.2&#8243;][et_pb_column type=&#8221;1_3&#8243;][et_pb_image src=&#8221;https:\/\/www.chem.s.u-tokyo.ac.jp\/campbell\/wp-content\/uploads\/sites\/7\/2021\/02\/GECO_series.png&#8221; animation=&#8221;left&#8221; sticky=&#8221;on&#8221; animation_style=&#8221;slide&#8221; animation_duration=&#8221;500ms&#8221; animation_intensity_slide=&#8221;10%&#8221; animation_direction=&#8221;left&#8221; show_bottom_space=&#8221;off&#8221; _builder_version=&#8221;3.12.2&#8243; \/][et_pb_text _builder_version=&#8221;3.12.2&#8243;]<\/p>\n<p style=\"text-align: left;\">3D representations of GECO series with different colors<\/p>\n<p>[\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;2_3&#8243;][et_pb_text admin_label=&#8221;Neural activity indicators (JP)&#8221; background_position=&#8221;top_left&#8221; background_repeat=&#8221;repeat&#8221; background_size=&#8221;initial&#8221; _builder_version=&#8221;3.12.2&#8243;]<\/p>\n<div id=\"stacks_out_198_page3\" class=\"stacks_out\" style=\"color: #000000;\">\n<div id=\"stacks_in_198_page3\" class=\"stacks_in text_stack\">\n<h3 style=\"color: #666666;\"><span style=\"color: #000000; font-size: 18pt;\"><strong>\u795e\u7d4c\u6d3b\u52d5\u3092\u53ef\u8996\u5316\u3059\u308b\u53ef\u8996\u5149\uff5e\u8fd1\u8d64\u5916\u86cd\u5149\u6307\u793a\u85ac\u306e\u958b\u767a<\/strong><\/span><\/h3>\n<div id=\"stacks_out_196_page3\" class=\"stacks_out\" style=\"color: #000000;\">\n<div id=\"stacks_in_196_page3\" class=\"stacks_in text_stack\">\n<p>\u30e2\u30c7\u30eb\u751f\u7269\u306b\u304a\u3051\u308b\u795e\u7d4c\u6d3b\u52d5\u306e\u53ef\u8996\u5316\u306f\u3001\u4eba\u9593\u306e\u8133\u6a5f\u80fd\u3084\u795e\u7d4c\u5909\u6027\u75be\u60a3\u3092\u7406\u89e3\u3059\u308b\u305f\u3081\u306e\u5f37\u529b\u306a\u624b\u6cd5\u306e\u4e00\u3064\u3067\u3059\u3002\u795e\u7d4c\u6d3b\u52d5\u3092\u53ef\u8996\u5316\u3059\u308b\u305f\u3081\u306b\u306f\u3001\u30a4\u30aa\u30f3\u6fc3\u5ea6\u3001\u819c\u96fb\u5727\u3001\u795e\u7d4c\u4f1d\u9054\u7269\u8cea\u306e\u5909\u5316\u3092\u691c\u51fa\u3059\u308b\u6307\u793a\u85ac\u304c\u5fc5\u8981\u3067\u3059\u3002\u30ad\u30e3\u30f3\u30d9\u30eb\u7814\u7a76\u5ba4\u306f\u3001GECO\u30b7\u30ea\u30fc\u30ba\u3068\u3057\u3066\u77e5\u3089\u308c\u308b\u8907\u6570\u306e\u8272\u306eCa<sup>2+<\/sup>\u6307\u793a\u85ac\u3092\u958b\u767a\u3057\u3001\u591a\u8272\u795e\u7d4c\u6d3b\u52d5\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u306e\u5206\u91ce\u3092\u7acb\u3061\u4e0a\u3052\u307e\u3057\u305f\u3002\u79c1\u305f\u3061\u306f\u307e\u305a\u8d64\u8272\u86cd\u5149\u3092\u6301\u3064R-GECO1\u3092\u5831\u544a\u3057\u3001\u3053\u308c\u306f\u4e16\u754c\u4e2d\u306e\u7814\u7a76\u5ba4\u304c\u3053\u308c\u307e\u3067\u306b\u767a\u8868\u3057\u3066\u3044\u308b\u4ed6\u306e\u591a\u304f\u306e\u8d64\u8272\u86cd\u5149\u6307\u793a\u85ac\u306e\u57fa\u790e\u3068\u306a\u3063\u3066\u3044\u307e\u3059\u3002GECO \u30b7\u30ea\u30fc\u30ba\u306f\u30012\u5149\u5b50\u304a\u3088\u3073\u30ec\u30b7\u30aa\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u306b\u6700\u9069\u306a\u3088\u3046\u306b\u3055\u3089\u306b\u62e1\u5f35\u3055\u308c\u307e\u3057\u305f\u3002R-GECO1\u304a\u3088\u3073\u305d\u306e\u6539\u826f\u7248\u306f\u3001\u4e16\u754c\u4e2d\u306e\u6570\u767e\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306b\u304a\u3044\u3066\u81ea\u7531\u306b\u4f7f\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002GECO\u30b7\u30ea\u30fc\u30ba\u306b\u6700\u8fd1\u8ffd\u52a0\u3055\u308c\u305f\u6307\u793a\u85ac\u3068\u3057\u3066\u3001\u8fd1\u8d64\u5916\uff08NIR\uff09-GECO1\u304a\u3088\u30732\u304c\u3042\u308a\u307e\u3059\u3002\u30ad\u30e3\u30f3\u30d9\u30eb\u7814\u7a76\u5ba4\u3067\u306f\u3001\u4ed6\u306b\u3082\u819c\u96fb\u5727\u6307\u793a\u85ac\u3067\u3042\u308b\u8fd1\u8d64\u5916\u86cd\u5149QuasAr\u30b7\u30ea\u30fc\u30ba\u3001\u8d64\u8272\u86cd\u5149FlicR1\u96fb\u5727\u6307\u793a\u85ac\u3001\u8d64\u8272\u86cd\u5149\u30b0\u30eb\u30bf\u30df\u30f3\u9178\u6307\u793a\u85ac\u306a\u3069\u3001\u8d64\u8272\uff5e\u8fd1\u8d64\u5916\u9818\u57df\u306e\u795e\u7d4c\u6d3b\u52d5\u6307\u793a\u85ac\u3092\u3044\u304f\u3064\u304b\u958b\u767a\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<\/div>\n<div class=\"stacks_in text_stack\">\n<div id=\"stacks_out_198_page3\" class=\"stacks_out\" style=\"color: #000000;\">\n<div id=\"stacks_in_198_page3\" class=\"stacks_in text_stack\">\n<p>&nbsp;<\/p>\n<\/div>\n<\/div>\n<div id=\"stacks_out_398_page3\" class=\"stacks_out\" style=\"color: #000000;\">\n<div class=\"stacks_in text_stack\"><\/div>\n<div class=\"stacks_in text_stack\"><\/div>\n<div id=\"stacks_in_398_page3\" class=\"stacks_in text_stack\"><strong>\u4ee3\u8868\u7684\u306a\u8ad6\u6587<\/strong><\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"stacks_out_290_page3\" class=\"stacks_out\" style=\"color: #000000;\">\n<div id=\"stacks_in_290_page3\" class=\"stacks_in text_stack\">\n<ul class=\"square\">\n<li>Y. Zhao, et al., \u201c<a href=\"https:\/\/science.sciencemag.org\/content\/333\/6051\/1888\" target=\"_blank\" rel=\"noopener\">An Expanded Palette of Genetically Encoded Ca<sup>2+<\/sup> Indicators<\/a>\u201d, <em>Science, <\/em>2011, <strong>333,<\/strong> 1888\u20131891.<\/li>\n<li>J. Wu, et al., \u201c<a href=\"https:\/\/www.nature.com\/articles\/ncomms6262\" target=\"_blank\" rel=\"noopener\">A long Stokes shift red fluorescent protein Ca<sup>2+<\/sup> indicator for 2-photon and ratiometric imaging<\/a>\u201d, <em>Nat. Commun., <\/em>2014, <strong>5,<\/strong> 5262.<\/li>\n<li>D.R. Hochbaum, et al., \u201c<a href=\"https:\/\/www.nature.com\/articles\/nmeth.3000\" target=\"_blank\" rel=\"noopener\">All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins<\/a>\u201d, <em>Nat. Methods, <\/em>2014, <strong>11,<\/strong> 825\u2013833.<\/li>\n<li>J. Wu, et al., \u201c<a href=\"https:\/\/pubs.acs.org\/doi\/abs\/10.1021\/acschembio.7b01085\" target=\"_blank\" rel=\"noopener\">Genetically Encoded Glutamate Indicators with Altered Color and Topology<\/a>\u201d, <em>ACS Chem. Biol.,<\/em> 2018, <strong>13,<\/strong> 1832\u20131837.<\/li>\n<li>Y. Qian, et al., \u201c<a href=\"https:\/\/www.nature.com\/articles\/s41592-018-0294-6\" target=\"_blank\" rel=\"noopener\">A genetically encoded near-infrared fluorescent calcium ion indicator<\/a>\u201d, <em>Nat. Methods, <\/em>2019, <strong>16,<\/strong> 171\u2013174.<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section bb_built=&#8221;1&#8243; fullwidth=&#8221;off&#8221; specialty=&#8221;off&#8221; background_color=&#8221;#ededed&#8221; inner_shadow=&#8221;off&#8221; parallax=&#8221;off&#8221; parallax_method=&#8221;css&#8221; prev_background_color=&#8221;#ffffff&#8221; next_background_color=&#8221;#ffffff&#8221;][et_pb_row][et_pb_column type=&#8221;1_3&#8243;][et_pb_image src=&#8221;https:\/\/www.chem.s.u-tokyo.ac.jp\/campbell\/wp-content\/uploads\/sites\/7\/2021\/02\/ddFP_PhoCl_2.png&#8221; animation=&#8221;left&#8221; sticky=&#8221;off&#8221; animation_style=&#8221;slide&#8221; animation_duration=&#8221;500ms&#8221; animation_intensity_slide=&#8221;10%&#8221; animation_direction=&#8221;left&#8221; _builder_version=&#8221;3.12.2&#8243; \/][et_pb_text _builder_version=&#8221;3.12.2&#8243;]<\/p>\n<p style=\"text-align: left;\">Schematic representation of A) ddFP and B) PhoCl<\/p>\n<p>[\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;2_3&#8243;][et_pb_text admin_label=&#8221;Innovative FP tools (JP)&#8221; background_position=&#8221;top_left&#8221; background_repeat=&#8221;repeat&#8221; background_size=&#8221;initial&#8221; _builder_version=&#8221;3.12.2&#8243;]<\/p>\n<div id=\"stacks_out_198_page3\" class=\"stacks_out\" style=\"color: #000000;\">\n<div id=\"stacks_in_198_page3\" class=\"stacks_in text_stack\">\n<h3><strong>\u6307\u5411\u6027\u9032\u5316\u306b\u3088\u308b\u9769\u65b0\u7684\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u30c4\u30fc\u30eb\u306e\u5275\u6210<\/strong><\/h3>\n<p>\u30ad\u30e3\u30f3\u30d9\u30eb\u7814\u7a76\u5ba4\u306f\u3001\u30b5\u30f3\u30b4\u7531\u6765\u306e\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u5909\u7570\u4f53\u306e\u9069\u7528\u7bc4\u56f2\u3092\u62e1\u5f35\u3057\u3001\u69d8\u3005\u306a\u5fdc\u7528\u5206\u91ce\u3067\u5229\u7528\u3067\u304d\u308b\u3088\u3046\u306b\u3057\u307e\u3057\u305f\u3002\u4f8b\u3048\u3070\u3001\u30b7\u30a2\u30f3\u3001\u9ec4\u8272\u3001\u7dd1\u304b\u3089\u8d64\u8272\u3078\u306e\u5149\u5909\u63db\uff08\uff1d\u5149\u7167\u5c04\u306b\u3088\u3063\u3066\u767a\u8272\u56e3\u306e\u8272\u304c\u5909\u308f\u308b\u3053\u3068\uff09\u53ef\u80fd\u306a\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u958b\u767a\u3057\u307e\u3057\u305f\u3002\u3053\u308c\u3089\u306e\u65b0\u3057\u3044\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u3001\u5358\u4e00\u7d30\u80de\u306b\u304a\u3051\u308b\u8907\u6570\u306e\u751f\u7269\u5b66\u7684\u30d7\u30ed\u30bb\u30b9\u306e\u540c\u6642\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u3084\u3001\u8d85\u89e3\u50cf\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u6280\u8853\u306e\u5411\u4e0a\u306a\u3069\u3001\u591a\u304f\u306e\u65b0\u3057\u3044\u5fdc\u7528\u3092\u53ef\u80fd\u306b\u3057\u307e\u3057\u305f\u3002\u305d\u306e\u5f8c\u3001\u30d8\u30c6\u30ed\u4e8c\u91cf\u4f53\u8907\u5408\u4f53\u3092\u5f62\u6210\u3059\u308b\u3053\u3068\u3067\u8f1d\u5ea6\u3092\u5897\u3059\u4e8c\u91cf\u4f53\u4f9d\u5b58\u6027\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\uff08ddFP\uff09\u3082\u958b\u767a\u3057\u307e\u3057\u305f\u3002\u3053\u306e\u9769\u65b0\u7684\u306a\u6280\u8853\u306f\u3001\u751f\u7d30\u80de\u3067\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u9593\u76f8\u4e92\u4f5c\u7528\u3092\u691c\u51fa\u3059\u308b\u305f\u3081\u306e\u5f37\u529b\u306a\u65b0\u624b\u6bb5\u3068\u306a\u3063\u3066\u3044\u307e\u3059\u3002\u6700\u8fd1\u3001\u30ad\u30e3\u30f3\u30d9\u30eb\u7814\u7a76\u5ba4\u306f\u3001\u5149\u3067\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6a5f\u80fd\u3092\u5236\u5fa1\u3059\u308b\u6839\u672c\u7684\u306b\u65b0\u3057\u3044\u5149\u907a\u4f1d\u5b66\u7684\u30c4\u30fc\u30eb\u3068\u3057\u3066\u3001\u5149\u5207\u65ad\u53ef\u80fd\u306a\u30bf\u30f3\u30d1\u30af\u8cea\uff08PhoCl\uff09\u3092\u958b\u767a\u3057\u307e\u3057\u305f\u3002\u7b2c\u4e00\u4e16\u4ee3\u3068\u7b2c\u4e8c\u4e16\u4ee3\u306ePhoCl\u306f\u3001\u5149\u306b\u3088\u308b\u8ee2\u5199\u6d3b\u6027\u5316\u3001\u907a\u4f1d\u5b50\u7d44\u307f\u63db\u3048\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u5c40\u5728\u306e\u64cd\u4f5c\u3001\u9175\u7d20\u6d3b\u6027\u306e\u6d3b\u6027\u5316\u306a\u3069\u306b\u4f7f\u7528\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n<p>&nbsp;<\/p>\n<\/div>\n<\/div>\n<div id=\"stacks_out_398_page3\" class=\"stacks_out\" style=\"color: #000000;\">\n<div class=\"stacks_in text_stack\"><\/div>\n<div class=\"stacks_in text_stack\"><\/div>\n<div id=\"stacks_in_398_page3\" class=\"stacks_in text_stack\"><strong>\u4ee3\u8868\u7684\u306a\u8ad6\u6587<br \/>\n<\/strong><\/div>\n<\/div>\n<ul class=\"square\">\n<li><span style=\"color: #000000;\">H. Hoi, et al., \u201c<a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0022283610006996?via%3Dihub\" target=\"_blank\" rel=\"noopener\">A Monomeric Photoconvertible Fluorescent Protein for Imaging of Dynamic Protein Localization<\/a>\u201d, <em>J. Mol. Biol., <\/em>2010, <strong>401,<\/strong> 776\u2013791.<\/span><\/li>\n<li><span style=\"color: #000000;\">H-w. Ai, et al., \u201c<a href=\"https:\/\/www.nature.com\/articles\/nprot.2014.054\" target=\"_blank\" rel=\"noopener\">Engineering and characterizing monomeric fluorescent proteins for live-cell imaging applications<\/a>\u201d.<em> Nat. Protocols,<\/em> 2014, <strong>9,<\/strong> 910\u2013928.<\/span><\/li>\n<li><span style=\"color: #000000;\">Y. Ding et al., \u201c<a href=\"https:\/\/www.nature.com\/articles\/nmeth.3261\" target=\"_blank\" rel=\"noopener\">Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange<\/a>\u201d, <em>Nature Methods,<\/em> 2015, <strong>12,<\/strong> 195\u2013198.<\/span><\/li>\n<li><span style=\"color: #000000;\">W. Zhang, et al., \u201c<a href=\"https:\/\/www.nature.com\/articles\/nmeth.4222\" target=\"_blank\" rel=\"noopener\">Optogenetic Control with a Photocleavable Protein, PhoCl<\/a>\u201d, <em>Nat. Methods,<\/em> 2017, <strong>14,<\/strong> 391-394.<\/span><\/li>\n<li><span style=\"color: #000000;\">X. Lu, et al., \u201c<a href=\"https:\/\/pubs.rsc.org\/en\/content\/articlelanding\/2021\/SC\/D1SC01059J\" target=\"_blank\" rel=\"noopener\">Photocleavable proteins that undergo fast and efficient dissociation<\/a>\u201d, <em>Chem. Sci.,<\/em> 2021, <strong>12,<\/strong> 9658-9672.<\/span><\/li>\n<\/ul>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section bb_built=&#8221;1&#8243; fullwidth=&#8221;off&#8221; specialty=&#8221;off&#8221; prev_background_color=&#8221;#ededed&#8221; next_background_color=&#8221;#ededed&#8221;][et_pb_row][et_pb_column type=&#8221;1_3&#8243;][et_pb_image src=&#8221;https:\/\/www.chem.s.u-tokyo.ac.jp\/campbell\/wp-content\/uploads\/sites\/7\/2021\/02\/iLacco_structure.png&#8221; animation_style=&#8221;slide&#8221; animation_duration=&#8221;500ms&#8221; animation_intensity_slide=&#8221;10%&#8221; animation_direction=&#8221;left&#8221; show_bottom_space=&#8221;off&#8221; _builder_version=&#8221;3.12.2&#8243; \/][et_pb_text _builder_version=&#8221;3.12.2&#8243;]<\/p>\n<p>Schematic and 3D structural representations of our lactate sensor (in collaboration with Dr. M. Joanne Lemieux and Dr. Yurong Wen, University of Alberta)<\/p>\n<p>[\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;2_3&#8243;][et_pb_text admin_label=&#8221;Metabolic sensors (JP)&#8221; background_position=&#8221;top_left&#8221; background_repeat=&#8221;repeat&#8221; background_size=&#8221;initial&#8221; _builder_version=&#8221;3.12.2&#8243;]<\/p>\n<div id=\"stacks_out_198_page3\" class=\"stacks_out\" style=\"color: #000000;\">\n<div id=\"stacks_in_198_page3\" class=\"stacks_in text_stack\">\n<div id=\"stacks_out_200_page3\" class=\"stacks_out\" style=\"color: #000000;\">\n<div id=\"stacks_in_200_page3\" class=\"stacks_in text_stack\">\n<h3 style=\"color: #666666;\"><span style=\"color: #000000;\"><strong>\u75be\u60a3\u306b\u304a\u3051\u308b\u751f\u7269\u5b66\u7684\u30a8\u30cd\u30eb\u30ae\u30fc\u6d88\u8cbb\u306e\u89e3\u660e<\/strong><\/span><\/h3>\n<p><span style=\"color: #000000;\">\u591a\u304f\u306e\u75be\u60a3\u306f\u3001\u7d30\u80de\u304c\u751f\u7269\u5b66\u7684\u30a8\u30cd\u30eb\u30ae\u30fc\u6e90\u306b\u30a2\u30af\u30bb\u30b9\u3057\u305f\u308a\u3001\u305d\u308c\u3092\u5229\u7528\u3057\u305f\u308a\u3059\u308b\u65b9\u6cd5\u306e\u5909\u5316\u306b\u95a2\u9023\u3057\u305f\u539f\u56e0\u3092\u6301\u3063\u3066\u3044\u307e\u3059\u3002\u91cd\u8981\u306a\u4f8b\u3068\u3057\u3066\u3001\u8133\u306e\u30a8\u30cd\u30eb\u30ae\u30fc\u4ee3\u8b1d\u306e\u5909\u5316\u3001\u7279\u306b\u4e73\u9178\u306e\u5229\u7528\u53ef\u80fd\u6027\u306e\u5909\u5316\u304c\u795e\u7d4c\u5909\u6027\u75be\u60a3\u3068\u7d50\u3073\u3064\u3044\u3066\u3044\u308b\u4e8b\u306a\u3069\u304c\u6319\u3052\u3089\u308c\u307e\u3059\u3002\u6b8b\u5ff5\u306a\u304c\u3089\u3001\u73fe\u5728\u3001\u7d30\u80de\u306e\u4ee3\u8b1d\u5168\u4f53\u3092\u8907\u6570\u30d1\u30e9\u30e1\u30fc\u30bf\u30fc\u3067\u53ef\u8996\u5316\u3059\u308b\u30c4\u30fc\u30eb\u304c\u306a\u3044\u305f\u3081\u30011\u3064\u306e\u7d30\u80de\u304c\u751f\u7269\u5b66\u7684\u30a8\u30cd\u30eb\u30ae\u30fc\u6e90\u306b\u95a2\u3059\u308b\u57fa\u672c\u7684\u306a\u4ee3\u8b1d\u7d4c\u8def\u306e\u305d\u308c\u305e\u308c\u3092\u3069\u306e\u7a0b\u5ea6\u5229\u7528\u3057\u3066\u3044\u308b\u304b\u3092\u53ef\u8996\u5316\u3059\u308b\u3053\u3068\u306f\u3067\u304d\u307e\u305b\u3093\u3002\u305d\u3053\u3067\u30ad\u30e3\u30f3\u30d9\u30eb\u7814\u7a76\u5ba4\u3067\u306f\u3001\u7d30\u80de\u306e\u4ee3\u8b1d\u7d4c\u8def\u306e\u5404\u91cd\u8981\u5206\u5b50\u306b\u5bfe\u3059\u308b\u9ad8\u6027\u80fd\u6307\u793a\u85ac\u306e\u958b\u767a\u3092\u901a\u3058\u3066\u3001\u7d30\u80de\u5185\u4ee3\u8b1d\u306e\u5168\u4f53\u50cf\u3092\u521d\u3081\u3066\u53ef\u8996\u5316\u3059\u308b\u3053\u3068\u3092\u76ee\u6307\u3057\u3066\u3044\u307e\u3059\u3002<\/span><\/p>\n<\/div>\n<\/div>\n<div id=\"stacks_out_403_page3\" class=\"stacks_out\" style=\"color: #000000;\">\n<div class=\"stacks_in text_stack\" style=\"color: #000000;\">\n<div id=\"stacks_out_198_page3\" class=\"stacks_out\" style=\"color: #000000;\">\n<div id=\"stacks_in_198_page3\" class=\"stacks_in text_stack\">\n<p>&nbsp;<\/p>\n<\/div>\n<\/div>\n<div id=\"stacks_out_398_page3\" class=\"stacks_out\" style=\"color: #000000;\">\n<div class=\"stacks_in text_stack\"><\/div>\n<div class=\"stacks_in text_stack\"><\/div>\n<div id=\"stacks_in_398_page3\" class=\"stacks_in text_stack\"><strong>\u4ee3\u8868\u7684\u306a\u8ad6\u6587<\/strong><\/div>\n<\/div>\n<\/div>\n<div id=\"stacks_in_403_page3\" class=\"stacks_in text_stack\">\n<ul class=\"square\" style=\"color: #000000;\">\n<li>Y. Zhao, et al., \u201c<a href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acscentsci.0c00518\">High performance intensiometric direct- and inverse-response genetically encoded biosensors for citrate<\/a>\u201d, <em>ACS Cent. Sci.,<\/em> 2020, <strong>6,<\/strong> 1441\u20131450.<\/li>\n<li>Y. Nasu, et al., &#8220;<a href=\"https:\/\/www.nature.com\/articles\/s41467-021-27332-2\" target=\"_blank\" rel=\"noopener\">A genetically encoded fluorescent biosensor for extracellular L-lactate<\/a>&#8220;, <em>Nat. Commun.,<\/em> 2021, <strong>12,<\/strong> 7058.<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section bb_built=&#8221;1&#8243; fullwidth=&#8221;off&#8221; specialty=&#8221;off&#8221; background_color=&#8221;#ededed&#8221; inner_shadow=&#8221;off&#8221; parallax=&#8221;off&#8221; parallax_method=&#8221;css&#8221; prev_background_color=&#8221;#ffffff&#8221;][et_pb_row][et_pb_column type=&#8221;1_3&#8243;][et_pb_image src=&#8221;https:\/\/www.chem.s.u-tokyo.ac.jp\/campbell\/wp-content\/uploads\/sites\/7\/2021\/02\/SNAPtag.png&#8221; animation_style=&#8221;slide&#8221; animation_duration=&#8221;500ms&#8221; animation_intensity_slide=&#8221;10%&#8221; animation_direction=&#8221;left&#8221; _builder_version=&#8221;3.12.2&#8243; \/][et_pb_text _builder_version=&#8221;3.12.2&#8243;]<\/p>\n<p style=\"text-align: left;\">Example of chemi-genetic fluorescent protein, where a synthetic dye (space-filling model) is attached to a tag protein (PDB: 6GA0)<\/p>\n<p>[\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;2_3&#8243;][et_pb_text admin_label=&#8221;Chemi-genetic (JP)&#8221; background_position=&#8221;top_left&#8221; background_repeat=&#8221;repeat&#8221; background_size=&#8221;initial&#8221; _builder_version=&#8221;3.12.2&#8243;]<\/p>\n<div id=\"stacks_out_198_page3\" class=\"stacks_out\" style=\"color: #000000;\">\n<div id=\"stacks_in_198_page3\" class=\"stacks_in text_stack\">\n<div id=\"stacks_out_200_page3\" class=\"stacks_out\" style=\"color: #000000;\">\n<div id=\"stacks_in_200_page3\" class=\"stacks_in text_stack\">\n<h3 style=\"color: #666666;\"><span style=\"color: #000000;\"><strong>\u5408\u6210\u5206\u5b50\u3068\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u8907\u5408\u306b\u3088\u308b\u5316\u5b66\u907a\u4f1d\u5b66\u6307\u793a\u85ac\u306e\u958b\u767a<\/strong><\/span><\/h3>\n<p style=\"color: #666666;\"><span style=\"color: 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src=&#8221;https:\/\/www.chem.s.u-tokyo.ac.jp\/campbell\/wp-content\/uploads\/sites\/7\/2021\/02\/GECO_series.png&#8221; animation=&#8221;left&#8221; sticky=&#8221;on&#8221; animation_style=&#8221;slide&#8221; animation_duration=&#8221;500ms&#8221; animation_intensity_slide=&#8221;10%&#8221; animation_direction=&#8221;left&#8221; show_bottom_space=&#8221;off&#8221; _builder_version=&#8221;3.12.2&#8243; \/][et_pb_text _builder_version=&#8221;3.12.2&#8243;] 3D representations of GECO series with different colors [\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;2_3&#8243;][et_pb_text admin_label=&#8221;Neural activity indicators (JP)&#8221; background_position=&#8221;top_left&#8221; background_repeat=&#8221;repeat&#8221; background_size=&#8221;initial&#8221; _builder_version=&#8221;3.12.2&#8243;] \u795e\u7d4c\u6d3b\u52d5\u3092\u53ef\u8996\u5316\u3059\u308b\u53ef\u8996\u5149\uff5e\u8fd1\u8d64\u5916\u86cd\u5149\u6307\u793a\u85ac\u306e\u958b\u767a 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&nbsp; \u4ee3\u8868\u7684\u306a\u8ad6\u6587 Y. Zhao, et al., \u201cAn Expanded Palette of Genetically Encoded Ca2+ Indicators\u201d, Science, 2011, 333, 1888\u20131891. J. Wu, et al., \u201cA long<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-2317","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/campbell.chem.s.u-tokyo.ac.jp\/index.php?rest_route=\/wp\/v2\/pages\/2317","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/campbell.chem.s.u-tokyo.ac.jp\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/campbell.chem.s.u-tokyo.ac.jp\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/campbell.chem.s.u-tokyo.ac.jp\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/campbell.chem.s.u-tokyo.ac.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2317"}],"version-history":[{"count":1,"href":"https:\/\/campbell.chem.s.u-tokyo.ac.jp\/index.php?rest_route=\/wp\/v2\/pages\/2317\/revisions"}],"predecessor-version":[{"id":2874,"href":"https:\/\/campbell.chem.s.u-tokyo.ac.jp\/index.php?rest_route=\/wp\/v2\/pages\/2317\/revisions\/2874"}],"wp:attachment":[{"href":"https:\/\/campbell.chem.s.u-tokyo.ac.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2317"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}