{"id":3018,"date":"2024-08-25T04:29:44","date_gmt":"2024-08-25T13:29:44","guid":{"rendered":"https:\/\/campbell.chem.s.u-tokyo.ac.jp\/?page_id=3018"},"modified":"2026-04-20T19:13:35","modified_gmt":"2026-04-21T04:13:35","slug":"publication","status":"publish","type":"page","link":"https:\/\/campbell.chem.s.u-tokyo.ac.jp\/?page_id=3018","title":{"rendered":"Publications"},"content":{"rendered":"\n

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Preprint. Ratiometric Fluorescent Protein Biosensors Reveal Citrate Dynamics and Cellular Heterogeneity<\/strong><\/h3>\n\n\n\n

S. Hario<\/strong> (equal contribution), N. Tamura (equal contribution), B.S. Alladin-Mustan<\/strong>, S. M. Ali, M.S. Macauley, Y. Shen<\/strong>, R.E. Campbell, I. Huppertz*, and K. Takahashi-Yamashiro<\/strong>*, \u201cRatiometric Fluorescent Protein Biosensors Reveal Citrate Dynamics and Cellular Heterogeneity\u201d, bioRxiv<\/em>, 2026.04.16.718871<\/a>.<\/p>\n\n\n\n

Preprint. A StayGold-based calcium ion indicator<\/strong><\/h3>\n\n\n\n

I. Miyazaki<\/strong>, K.K. Tsao<\/strong>, T. Terai<\/strong>, K. Takahashi-Yamashiro<\/strong>, and R.E. Campbell*, \u201cA StayGold-based calcium ion indicator\u201d, bioRxiv, <\/em>2026.03.06.710044<\/a>.<\/p>\n\n\n\n

Preprint. Development of a photostable pH biosensor based on mStayGold<\/strong><\/h3>\n\n\n\n

M. Chang<\/strong>, K. Takahashi-Yamashiro<\/strong>, T. Terai<\/strong>, R.E. Campbell*, Kelvin K. Tsao<\/strong>*, \u201cDevelopment of a photostable pH biosensor based on mStayGold\u201d, bioRxiv<\/em>, 2026.03.06.710027<\/a>.<\/p>\n\n\n\n

Preprint. A sensitive orange fluorescent calcium ion indicator for imaging neural activity<\/strong><\/h3>\n\n\n\n

A. Aggarwal, H.A. Baker<\/strong>, C.D. D\u00fcrst, I-W. Chen, P. de Chambrier, J.M. Gonzales, J.S. Marvin, M. Vandal, T. Lundberg, K. Sakoi<\/strong>, R. Patel, C-Y. Wang, F. Visser, Y. Fouad, S. Sunil, M. Wiens<\/strong>, T. Terai<\/strong>, K. Takahashi-Yamashiro<\/strong>, R.J. Thompson, T.A. Brown, Y. Nasu, M.D. Nguyen, G.R.J. Gordon, S. McFarlane, K. Podgorski, A. Holtmaat, R.E. Campbell and A.W. Lohman*, \u201cA sensitive orange fluorescent calcium ion indicator for imaging neural activity\u201d, submitted<\/strong>. Preprint posted to bioRxiv<\/em> 2025.07.28.667269<\/a>.<\/p>\n\n\n\n

Preprint. High-performance genetically-encoded green and red fluorescent biosensors for pyruvate<\/strong><\/h3>\n\n\n\n

S. Imai<\/strong> (equal contribution), S. Hario<\/strong> (equal contribution), C. Suerte<\/strong>, I. Yamaguchi,<\/strong> K. Sakoi<\/strong>, T. Terai<\/strong>, K. Takahashi-Yamashiro<\/strong>, and R.E. Campbell*, \u201cHigh-performance genetically-encoded green and red fluorescent biosensors for pyruvate\u201d. Preprint posted to bioRxiv<\/em> 2025.04.17.649293<\/a>. <\/p>\n\n\n\n

Preprint. PinkyCaMP: a mScarlet-based calcium sensor with exceptional brightness, photostability, and multiplexing capabilities<\/strong><\/h3>\n\n\n\n

R. Fink (equal contribution), S. Imai <\/strong>(equal contribution), N. Gockel, G. Lauer, K. Renken, J. Wietek, P.J. Lamothe-Molina, F. Furhmann, M. Mittag, T. Ziebarth, A. Canziani, M. Kubitschke, V. Kistmacher, A. Kretschmer, E. Sebastian, D. Schmitz, T. Terai<\/strong>, J. Gruendemann, S. Hassan, T. Patriarchi, A. Reiner, M. Fuhrmann, R.E. Campbell, and O.A. Masseck*, \u201cPinkyCaMP: a mScarlet-based calcium sensor with exceptional brightness, photostability, and multiplexing capabilities\u201d, submitted<\/strong>. Preprint posted to bioRxiv<\/em> 2024.12.16.628673<\/a>.<\/p>\n\n\n\n

157. A red fluorescent genetically encoded biosensor for in vivo imaging of extracellular L-lactate dynamics<\/strong><\/h3>\n\n\n\n

Y. Kamijo<\/strong>, P. M\u00e4chler (equal contribution), N. Ness (equal contribution), C.Q. Vu (equal contribution), T. Kusakizako (equal contribution), J. Mannuthodikayil, Z. Ku, M. Boisvert, E. Grebenik, I. Miyazaki<\/strong>, R. Hashizume<\/strong>, H. Sato, R. Liu, Y. Hori, T. Tomita, T. Katayama, A. Furube, G. Caraveo, M-E. Paquet, M. Drobizhev, O.Nureki, S.Arai, M. Brancaccio, R.E. Campbell, D. Kleinfeld, and Y. Nasu<\/strong>*, \u201cA red fluorescent genetically encoded biosensor for in vivo imaging of extracellular L-lactate dynamics<\/a>\u201d, Nat. Commun.<\/em>, 2025, 16<\/strong>, 9531. Preprint posted to bioRxiv<\/em> 2022.08.30.505811<\/a>. <\/p>\n\n\n\n

156. Bipartite Genetically Encoded Biosensors to Sense Calcium Ion Dynamics at Membrane-Membrane Contact Sites<\/strong><\/h3>\n\n\n\n

I. Yamaguchi <\/strong>(equal contribution), L. Barazzuol (equal contribution), G. Dematteis, W. Zhu<\/strong>, Y. Wen, M. Drobizhev, D. Lim, R.E. Campbell, T. Cal\u00ec* and Y. Nasu<\/strong>*, \u201cBipartite Genetically Encoded Biosensors to Sense Calcium Ion Dynamics at Membrane-Membrane Contact Sites<\/a>\u201d, Anal. Chem.,<\/em> 2025, 97<\/strong>, 19848\u201319861.<\/p>\n\n\n\n

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155. Far-red fluorescent genetically encoded calcium ion indicators<\/strong><\/h3>\n\n\n\n

R. Dalangin<\/strong>, B.Z. Jia, Y. Qi, A. Aggarwal<\/strong>, K. Sakoi<\/strong>, M. Drobizhev, R.S. Molina, R. Patel, A.S. Abdelfattah, J. Zheng, D. Reep, J.P. Hasseman, The GENIE Project Team, Y. Zhao<\/strong>, J. Wu<\/strong>, K. Podgorski, A.G. Tebo, E.R. Schreiter, T.E. Hughes, T. Terai<\/strong>, M-E. Paquet, S.G. Megason, A.E. Cohen, Y. Shen<\/strong>*, and R.E. Campbell*, \u201cFar-red fluorescent genetically encoded calcium ion indicators<\/a>\u201d, Nat. Commun.<\/em>, 2025, 16<\/strong>, 3318. Preprint posted to bioRxiv <\/em>2020.11.12.380089<\/a>.<\/p>\n\n\n\n

154. Synthesis and application of a photocaged-L-lactate for studying the biological roles of L-lactate<\/strong><\/h3>\n\n\n\n

I. Miyazaki<\/strong>, K.K. Tsao*<\/strong>, Y. Kamijo<\/strong>, Y. Nasu<\/strong>, T. Terai*<\/strong>, R.E. Campbell*, \u201cSynthesis and application of a photocaged-L-lactate for studying the biological roles of L-lactate<\/a>\u201d, Commun. Chem<\/em>., 2025, 8<\/strong>, 104. Preprint posted to bioRxiv<\/em> 2024.01.30.577898<\/a>.<\/p>\n\n\n\n

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153. Astrocyte Kir4.1 expression level territorially controls excitatory transmission in the brain<\/strong><\/h3>\n\n\n\n

O. Tyurikova*, O. Kopach, K. Zheng, D. Rathore, N. Codadu, S-Y. Wu<\/strong>, Y. Shen<\/strong>, R.E. Campbell, R.C. Wykes, K. Volynski, L.P. Savtchenko, and D.A. Rusakov*, \u201cAstrocyte Kir4.1 expression level territorially controls excitatory transmission in the brain<\/a>\u201d, Cell Rep., <\/em>2025, <\/em>44<\/strong>, 115299.<\/p>\n\n\n\n

152. A chemigenetic indicator based on a synthetic chelator and a green fluorescent protein for imaging of intracellular sodium ion<\/strong><\/h3>\n\n\n\n

S. Takeuchi<\/strong>, S. Imai<\/strong>, T. Terai<\/strong>*, and R.E. Campbell*, \u201cA chemigenetic indicator based on a synthetic chelator and a green fluorescent protein for imaging of intracellular sodium ion<\/a>\u201d, RSC Chem. Biol., <\/em>2025, 6<\/strong>, 170-174.<\/p>\n\n\n\n

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151. Multimodal fluorescence-optoacoustic in vivo imaging of the near-infrared calcium ion indicator NIR-GECO2G<\/strong><\/span><\/h3>\n\n\n\n

S.F Shaykevich, J.P. Little, Y. Qian<\/strong>, M-E. Paquet, R.E. Campbell*, D. Razansky*, and S. Shoham*, \u201cMultimodal fluorescence-optoacoustic in vivo imaging of the near-infrared calcium ion indicator NIR-GECO2G<\/a>\u201d, Photoacoustics<\/em>, 2025, 41<\/strong>, 100671.<\/p>\n\n\n\n

150. A toolbox for ablating excitatory and inhibitory synapses<\/strong><\/h3>\n\n\n\n

A. Bareghamyan, C. Deng, S. Daoudi, S.C. Yadav, X. Lu<\/strong>, W. Zhang<\/strong>, R.E. Campbell, R.H. Kramer, D.M. Chenoweth, and D.B Arnold*, \u201cA toolbox for ablating excitatory and inhibitory synapses<\/a>\u201d, eLife<\/em>, 2024, 13<\/strong>, RP103757. Preprint posted to bioRxiv<\/em> 2024.09.23.614589<\/a>.<\/p>\n\n\n\n

149. High-performance chemigenetic potassium ion indicator<\/strong><\/h3>\n\n\n\n

D. Cheng<\/strong>, Z. Ouyang, X. He, Y. Nasu<\/strong>, Y. Wen, T. Terai<\/strong>*, and R.E. Campbell*, \u201cHigh-performance chemigenetic potassium ion indicator<\/a>\u201d, J. Am. Chem. Soc.<\/em>, 2024, 146<\/strong>, 35117-35128.<\/p>\n\n\n\n

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148. High-Throughput Discovery of Substrate Peptide Sequences for E3 Ubiquitin Ligases Using a cDNA Display Method<\/strong><\/h3>\n\n\n\n

K. Tamagawa<\/strong>, R.E. Campbell, and T. Terai<\/strong>*, \u201cHigh-Throughput Discovery of Substrate Peptide Sequences for E3 Ubiquitin Ligases Using a cDNA Display Method<\/span><\/a>\u201d, ChemBioChem<\/em>, 2024, 25<\/strong>, e202400617.<\/p>\n\n\n\n

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147. The best of both worlds: Chemigenetic fluorescent sensors for biological imaging<\/strong><\/h3>\n\n\n\n

K.K. Tsao<\/strong>* (equal contribution), S. Imai <\/strong>(equal contribution), M. Chang<\/strong>, S. Hario, T. Terai*, <\/strong>and R.E. Campbell*, \u201cThe best of both worlds: Chemigenetic fluorescent sensors for biological imaging<\/a>\u201d, Cell Chem. Biol.<\/em>, 2024, 31<\/strong>, 1652-1664.<\/p>\n\n\n\n

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146. A lactate-dependent shift of glycolysis mediates synaptic and cognitive processes in male mice<\/strong><\/h3>\n\n\n\n

I. Fern\u00e1ndez-Moncada*, G. Lavanco, U.B. Fundazuri, N. Bollmohr, S. Mountadem, T. Dalla Tor, P. Hachaguer, F. Julio-Kalajzic, D. Gisquet, R. Serrat, L. Bellocchio, A. Cannich, B. Fortunato-Marsol, Y. Nasu<\/strong>, R.E. Campbell, F. Drago, C. Cannizzaro, G. Ferreira, A-K. Bouzier-Sore, L. Pellerin, J.P. Bola\u00f1os, G. Bonvento, L.F. Barros, S.H.R. Oliet, A. Panatier, and G. Marsicano*, \u201cA lactate-dependent shift of glycolysis mediates synaptic and cognitive processes in male mice<\/a>\u201d, Nat. Commun<\/em>., 2024, 15<\/strong>, 6842. Preprint posted to bioRxiv<\/em> 2023.03.15.532748<\/a>.<\/p>\n\n\n\n

145. An automated screening platform for improving the performance of genetically encoded Ca2+<\/sup> biosensors in mammalian cells<\/strong><\/h3>\n\n\n\n

Y. Zhao<\/strong>*, Y. Shen<\/strong>, T. Veres, and R.E. Campbell*, “An automated screening platform for improving the performance of genetically encoded Ca2+<\/sup> biosensors in mammalian cells<\/a>\u201d, Sens. Diagn., <\/em>2024, 3<\/strong>, 1494-1504.<\/p>\n\n\n\n

144. Development of an miRFP680-Based Fluorescent Calcium Ion Biosensor Using End-Optimized Transposons<\/strong><\/h3>\n\n\n\n

F. Chai<\/strong>, H. Fujii (equal contribution), G.N.T. Le (equal contribution), C. Lin (equal contribution), K. Ota (equal contribution), K.M. Lin, L.M.T. Pham, P. Zou, M. Drobizhev, Y. Nasu<\/strong>, T. Terai<\/strong>, H. Bito, and R.E. Campbell*, \u201cDevelopment of an miRFP680-Based Fluorescent Calcium Ion Biosensor Using End-Optimized Transposons<\/a>\u201d, ACS Sens.<\/em>, 2024, 9<\/strong>, 3394\u20133402.<\/p>\n\n\n\n

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143. An optogenetic method for the controlled release of single molecules<\/strong><\/h3>\n\n\n\n

P. Kashyap, S. Bertelli, F. Cao, Y. Kostritskaia, F. Blank, N.A. Srikanth, C. Schlack-Leigers, R. Saleppico, D. Bierhuizen, X. Lu<\/strong>, W. Nickel, R.E. Campbell, A.J.R. Plested, T. Stauber, M.J. Taylor, and H. Ewers*, \u201cAn optogenetic method for the controlled release of single molecules<\/a>\u201d, Nat. Methods<\/em>, 2024, 21<\/strong>, 666\u2013672. Preprint posted to bioRxiv<\/em> 2023.09.16.557871<\/a>.<\/p>\n\n\n\n

142. A blue-shifted genetically encoded Ca2+ <\/sup>indicator with enhanced two-photon absorption<\/strong><\/h3>\n\n\n\n

A. Aggarwal<\/strong>, S. Sunil (equal contribution), I. Bendifallah (equal contribution), M. Moon<\/strong>, M. Drobizhev, L. Zarowny<\/strong>, J. Zheng, S.-Y. Wu<\/strong>, A.W. Lohman, A.G. Tebo, V. Emiliani, K. Podgorski, Y. Shen<\/strong>*, and R.E. Campbell*, \u201cA blue-shifted genetically encoded Ca2+ <\/sup>indicator with enhanced two-photon absorption<\/a>\u201d, Neurophoton<\/em>., 2024, 11, 024207. Preprint posted to bioRxiv<\/em> 2023.10.12.562058<\/a>.<\/p>\n\n\n\n

141. High performance genetically-encoded green fluorescent biosensors for intracellular L-lactate<\/strong><\/h3>\n\n\n\n

S. Hario<\/strong> (equal contribution), G.N.T. Le<\/strong> (equal contribution), H. Sugimoto, K. Takahashi-Yamashiro<\/strong>, S. Nishinami, H. Toda, S. Li<\/strong>, J.S. Marvin, S. Kuroda, M. Drobizhev, T. Terai<\/strong>, Y. Nasu<\/strong>*, and R.E. Campbell*, \u201cHigh performance genetically-encoded green fluorescent biosensors for intracellular L-lactate<\/a>\u201d, ACS Cent. Sci<\/em>., 2024, 10<\/strong>, 402\u2013416. Preprint posted to bioRxiv<\/em> 2022.10.19.512892<\/a>.<\/p>\n\n\n\n

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140. \u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u30bf\u30b0\u30bf\u30f3\u30d1\u30af\u8cea [Fluorescent proteins and protein tags]<\/strong><\/h3>\n\n\n\n

J. Adachi, T. Terai<\/strong>, S. Hario<\/strong>, R.E. Campbell, and Y. Hori, \u201c\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u30bf\u30b0\u30bf\u30f3\u30d1\u30af\u8cea (Fluorescent proteins and protein tags)\u201d, \u73fe\u4ee3\u5316\u5b66 (Chemistry Today<\/em>), 2024, 640<\/strong>, 33-39. (Not peer-reviewed)<\/p>\n\n\n\n

139. \u30bf\u30f3\u30d1\u30af\u8cea\u3092\u57fa\u76e4\u3068\u3059\u308b\u86cd\u5149\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u30bb\u30f3\u30b5\u30fc\u958b\u767a\u306e\u6700\u524d\u7dda [Development of fluorescent imaging sensors based on proteins]<\/strong><\/h3>\n\n\n\n

\u91dd\u5c3e \u7d17\u5f69<\/strong>, \u7af9\u5185 \u5fd7\u7e54<\/strong>, \u30ad\u30e3\u30f3\u30d9\u30eb \u30ed\u30d0\u30fc\u30c8 \u30a2\u30fc\u30eb*, \u5bfa\u4e95 \u7422\u4e5f<\/strong>*, \u201c\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u57fa\u76e4\u3068\u3059\u308b\u86cd\u5149\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u30bb\u30f3\u30b5\u30fc\u958b\u767a\u306e\u6700\u524d\u7dda<\/a>\u201c, \u65e5\u672c\u85ac\u7406\u5b66\u96d1\u8a8c, 2024, 159<\/strong>, 25-30. [Original citation] <\/strong><\/p>\n\n\n\n

S. Hario<\/strong>, S. Takeuchi<\/strong>, R.E. Campbell*, and T. Terai<\/strong>*, \u201cDevelopment of fluorescent imaging sensors based on proteins<\/a>\u201d, Folia Pharmacol. Jpn.<\/em>, 2024, 159<\/strong>, 25-30. [Translated citation]<\/strong><\/p>\n\n\n\n

138. A monochromatically excitable green-red dual-fluorophore fusion incorporating a new large Stokes shift fluorescent protein<\/strong><\/h3>\n\n\n\n

J.O. Ejike (equal contribution), M. Sadoine (equal contribution), Y. Shen<\/strong> (equal contribution), Y. Ishikawa (equal contribution), E. Sunal, S. H\u00e4nsch, A.B. Hamacher, W.B. Frommer*, M.M. Wudick, R.E. Campbell*, and T.J. Kleist, \u201cA monochromatically excitable green-red dual-fluorophore fusion incorporating a new large Stokes shift fluorescent protein<\/a>\u201d, Biochemistry<\/em>, 2024, 63<\/strong>, 171\u2013180. Preprint posted to bioRxiv<\/em> 2023.07.16.549156<\/a>.<\/p>\n\n\n\n

137. Development of general-purpose fluorescent biosensors for visualization of intracellular disease-related proteins (\u7d30\u80de\u5185\u75be\u60a3\u95a2\u9023\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u53ef\u8996\u5316\u3059\u308b\u6c4e\u7528\u7684\u86cd\u5149\u30bb\u30f3\u30b5\u30fc\u306e\u958b\u767a)<\/strong><\/h3>\n\n\n\n

R.E. Campbell, \u201cDevelopment of general-purpose fluorescent biosensors for visualization of intracellular disease-related proteins (\u7d30\u80de\u5185\u75be\u60a3\u95a2\u9023\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u53ef\u8996\u5316\u3059\u308b\u6c4e\u7528\u7684\u86cd\u5149\u30bb\u30f3\u30b5\u30fc\u306e\u958b\u767a)<\/a>\u201d, Nakatani Foundation Annual Report<\/em>, 2023, 37<\/strong>, 60-67. (Describes research performed by Issei Yamaguchi; published online January 2025; not peer-reviewed).<\/p>\n\n\n\n

136. Lactate biosensors for spectrally and spatially multiplexed fluorescence imaging<\/strong><\/h3>\n\n\n\n

Y. Nasu<\/strong>*, A. Aggarwal, G.N.T. Le<\/strong>, C.T. Vo, Y. Kambe, X. Wang, F.R.M. Beinlich, A.B. Lee, T.R. Ram, F. Wang, K.A. Gorzo, Y. Kamijo<\/strong>, M. Boisvert, S. Nishinami, G. Kawamura, T. Ozawa, H. Toda, G.R. Gordon, S. Ge, H. Hirase, M. Nedergaard, M.-E. Paquet, M. Drobizhev, K. Podgorski, and R.E. Campbell*, \u201cLactate biosensors for spectrally and spatially multiplexed fluorescence imaging<\/a>\u201d, Nat. Commun<\/em>., 2023, 14<\/strong>, 6598. Preprint posted to bioRxiv<\/em> 2022.12.27.522013<\/a>.<\/p>\n\n\n\n

135. Construction of the lactate-sensing fibermats by confining sensor fluorescent protein of lactate inside nanofibers of the poly(HPMA\/DAMA)\/ADH-nylon 6 core-shell fibremat<\/strong><\/h3>\n\n\n\n

Y. Kato, S. Iwata, Y. Nasu<\/strong>, A. Obata, K. Nagata, R.E. Campbell, and T. Mizuno, \u201cConstruction of the lactate-sensing fibermats by confining sensor fluorescent protein of lactate inside nanofibers of the poly(HPMA\/DAMA)\/ADH-nylon 6 core-shell fibremat<\/a>\u201d, RSC Adv.<\/em>, 2023,13<\/strong>, 29584-29593.<\/p>\n\n\n\n

134. Rational engineering of an improved genetically encoded pH sensor based on superecliptic pHluorin<\/strong><\/h3>\n\n\n\n

Y. Shen*<\/strong>, Y. Wen, S. Sposini, A.A. Vishwanath, A.S. Abdelfattah, E.R. Schreiter, M.J. Lemieux, J. de Juan-Sanz, D. Perrais, and R.E. Campbell*, \u201dRational engineering of an improved genetically encoded pH sensor based on superecliptic pHluorin<\/a>\u201d, ACS Sens<\/em>., 2023, 8<\/strong>, 3014\u20133022.<\/p>\n\n\n\n

133. Maximizing the performance of fluorescent protein-based biosensors<\/strong><\/h3>\n\n\n\n

F. Chai<\/strong>, D. Cheng<\/strong>, Y. Nasu<\/strong>*, T. Terai<\/strong>*, and R.E. Campbell*, \u201cMaximizing the performance of fluorescent protein-based biosensors<\/a>\u201d, Biochem. Soc. Trans<\/em>., 2023, 51<\/strong>, 1585\u20131595.<\/p>\n\n\n\n

132. Directed Evolution of a Genetically Encoded Bioluminescent Ca2+<\/sup> Sensor<\/strong><\/h3>\n\n\n\n

Y. Zhao, S. Lee, R.E. Campbell, M.Z. Lin*, \u201cDirected Evolution of a Genetically Encoded Bioluminescent Ca2+<\/sup> Sensor<\/a>\u201d, Eng. Proc<\/em>. 2023, 35<\/strong>, 20. (Not peer-reviewed)<\/p>\n\n\n\n

131. Biosensor optimization using a FRET pair based on mScarlet red fluorescent protein and an mScarlet-derived green fluorescent protein<\/strong><\/h3>\n\n\n\n

K. Gohil<\/strong>, S-Y. Wu<\/strong>, K. Takahashi-Yamashiro<\/strong>, Y. Shen<\/strong>*, and R.E. Campbell*, \u201cBiosensor optimization using a FRET pair based on mScarlet red fluorescent protein and an mScarlet-derived green fluorescent protein<\/a>\u201c, ACS Sens.<\/em>, 2023, 8,<\/strong> 587\u2013597. Preprint posted to bioRxiv<\/em> 2022.06.20.496847<\/a>.<\/p>\n\n\n\n

130. Chemigenetic indicators based on synthetic chelators and green fluorescent protein<\/strong><\/h3>\n\n\n\n

W. Zhu<\/strong>, S. Takeuchi<\/strong>, S. Imai<\/strong>, T. Terada, T. Ueda, Y. Nasu<\/strong>, T. Terai<\/strong>* and R.E. Campbell*, \u201cChemigenetic indicators based on synthetic chelators and green fluorescent protein<\/a>\u201d, Nat. Chem. Biol., <\/em>2023, 19<\/strong>, 38\u201344.<\/p>\n\n\n\n

Supplementary Information<\/a><\/p>\n\n\n\n

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129. A genetically-encoded far-red fluorescent calcium ion biosensor derived from a biliverdin-binding protein<\/strong><\/h3>\n\n\n\n

R. Hashizume<\/strong>, H. Fujii (equal contribution), S. Mehta (equal contribution), K. Ota (equal contribution), Y. Qian<\/strong>, W. Zhu<\/strong>, M. Drobizhev, Y. Nasu*<\/strong>, J. Zhang, H. Bito, and R.E. Campbell*, \u201cA genetically-encoded far-red fluorescent calcium ion biosensor derived from a biliverdin-binding protein<\/a>\u201d, Protein Sci.,<\/em> 2022, 31<\/strong>, e4440.<\/p>\n\n\n\n

Supporting Information<\/a><\/p>\n\n\n\n

128. The Endoplasmic Reticulum (ER) Kinase PERK Requires the Oxidoreductase ERO1 to Metabolically Adapt Mitochondria<\/strong><\/h3>\n\n\n\n

A. Bassot, J. Chen, K. Takahashi-Yamashiro<\/strong>, M.C. Yap, C.S. Gibhardt, G.N.T. Le<\/strong>, S. Hario<\/strong>, Y. Nasu<\/strong>, J. Moore, T. Guti\u00e9rrez, L. Mina, H. Mast, A. Moses, K. Ballanyi, H. Lemieux, R. Sitia, E. Zito, I. Bogeski, R.E. Campbell, and T. Simmen*, \u201cThe Endoplasmic Reticulum (ER) Kinase PERK Requires the Oxidoreductase ERO1 to Metabolically Adapt Mitochondria<\/a>\u201d, Cell Rep.,<\/em> 2022, 42<\/strong>, 111899.<\/p>\n\n\n\n

127. Quantification of intracellular citrate concentrations with genetically encoded biosensors<\/strong><\/h3>\n\n\n\n

Y. Zhao<\/strong>*, K. Takahashi-Yamashiro<\/strong>, Y. Shen<\/strong>, and R.E. Campbell*, \u201cQuantification of intracellular citrate concentrations with genetically encoded biosensors<\/a>\u201d, In: M. Sharma (Eds), Fluorescent Proteins. Methods in Molecular Biology<\/em><\/a>, vol. 2564<\/strong>, Humana, New York, NY, September 2022, pages 247-258. [ISBN: 978-1-0716-2666-5]<\/p>\n\n\n\n

126. A sensitive and specific genetically-encoded potassium ion biosensor for in vivo applications across the tree of life<\/strong><\/h3>\n\n\n\n

S-Y. Wu<\/strong>, Y. Wen, N.B.C. Serre, C.C.H. Laursen, A.G. Dietz, B.R. Taylor, M. Drobizhev, R.S. Molina, A. Aggarwal, V. Rancic, M. Becker, K. Ballanyi, K. Podgorski, H. Hirase, M. Nedergaard, M. Fendrych, M.J. Lemieux, D.F. Eberl, A.R. Kay, R.E. Campbell*, and Y. Shen<\/strong>*, \u201cA sensitive and specific genetically-encoded potassium ion biosensor for in vivo applications across the tree of life<\/a>\u201d, PLOS Biol.<\/em>, 2022, 20<\/strong>, e3001772. Preprint posted to bioRxiv<\/em> 2021.10.07.463410.<\/a><\/p>\n\n\n\n

125. Neurophotonic tools for microscopic measurements and manipulation: status report<\/strong><\/h3>\n\n\n\n

A. Abdelfattah, S. Ahuja, T. Akkin, S.R. Allu, J. Brake, D.A. Boas, E.M. Buckley, R.E. Campbell, A.I. Chen, X. Cheng, T. \u010ci\u017em\u00e1r, I. Costantini, M. De Vittorio, A. Devor*, P.R. Doran, M. El Khatib, V. Emiliani, N. Fomin-Thunemann, A. Gilad, S. Fainman, T. Fernandez-Alfonso, C.G.L. Ferri, X. Han, A. Harris, E.M.C. Hillman, U. Hochgeschwender, M.G. Holt, N. Ji, K. K\u0131l\u0131\u00e7, E. Lake, L. Li, T. Li, P. Machler, E.W. Miller, R.C. Mesquita, K. M. N. S. Nadella, U. Valentin N\u00e4gerl, Y. Nasu<\/strong>, A. Nimmerjahn, P. Ondr\u00e1ckov\u00e1, F.S. Pavone, C.P. Campos, D. Peterka, F. Pisano, F. Pisanello, F. Puppo, B.L. Sabatini, S. Sadegh, S. Sakadzic, S. Shoham, S.N. Shroff, R.A. Silver, R.R. Sims, S.L. Smith, V.J. Srinivasan, M. Thunemann, L. Tian, L. Tian, T. Troxler, A. Valera, A. Vaziri, S.A. Vinogradov, F. Vitale, L.V. Wang, H. Uhl\u00ed\u0159ov\u00e1, C. Xu, C. Yang, M-H. Yang, G. Yellen, O. Yizhar, and Y. Zhao, \u201cNeurophotonic tools for microscopic measurements and manipulation: status report<\/a>\u201d, Neurophoton.<\/em>, 2022, 9<\/strong> (S1), 013001.<\/p>\n\n\n\n

124. Cyan fluorescent proteins derived from mNeonGreen<\/strong><\/h3>\n\n\n\n

L. Zarowny,<\/strong> D. Clavel (equal contribution), R. Johannson (equal contribution), K. Duarte (equal contribution), H. Depernet (equal contribution), J. Dupuy, H. Baker<\/strong>, A. Brown, A. Royant, and R.E. Campbell*, \u201cCyan fluorescent proteins derived from mNeonGreen<\/a>\u201d, Protein Eng. Des. Sel<\/em>., 2022, 35<\/strong>, gzac004.<\/p>\n\n\n\n

123. Fluorescent indicators for biological imaging of monatomic ions<\/strong><\/h3>\n\n\n\n

S-Y. Wu<\/strong>, Y. Shen<\/strong>, I. Shkolnikov<\/strong> and R.E. Campbell*, \u201cFluorescent indicators for biological imaging of monatomic ions<\/a>\u201d, Front. Cell Dev. Biol<\/em>., 2022, 10<\/strong>, 885440<\/p>\n\n\n\n

122. Absolute measurement of cellular activities using photochromic single-fluorophore biosensors and intermittent quantification<\/strong><\/h3>\n\n\n\n

F. Bierbuesse, A.C. Bourges, V. Gielen, V. M\u00f6nkem\u00f6ller, W. Vandenberg, Y. Shen<\/strong>, J. Hofkens, P. Vanden Berghe, R.E. Campbell, B. Moeyaert, and P. Dedecker*, \u201cAbsolute measurement of cellular activities using photochromic single-fluorophore biosensors and intermittent quantification<\/a>\u201d, Nat. Commun<\/em>., 2022, 13<\/strong>, 1850. Preprint posted to bioRxiv<\/em> 2020.10.29.360214<\/a>.<\/p>\n\n\n\n

121. Live cell tracking of macrophage efferocytosis during Drosophila embryo development in vivo<\/strong><\/h3>\n\n\n\n

M.H. Raymond (equal contribution), A.J. Davidson (equal contribution), Y. Shen<\/strong>, D.R. Tudor, C.D. Lucas, S. Morioka, J.S.A. Perry, J. Krapivkina, D. Perrais, L.J. Schumacher, R.E. Campbell, W. Wood*, and K.S. Ravichandran*, \u201cLive cell tracking of macrophage efferocytosis during Drosophila embryo development in vivo<\/a>\u201d, Science<\/em>, 2022, 375<\/strong>, 1182-1187.<\/p>\n\n\n\n

120. Barcodes, co-cultures, and deep learning take genetically encoded biosensor multiplexing to the nth degree<\/strong><\/h3>\n\n\n\n

T. Terai<\/strong> and R.E.Campbell*, \u201cBarcodes, co-cultures, and deep learning take genetically encoded biosensor multiplexing to the nth degree<\/a>\u201d, Mol. Cell<\/em>, 2022, 82<\/strong>, 239-240. [not peer reviewed]<\/p>\n\n\n\n

119. A genetically encoded fluorescent biosensor for extracellular L-lactate<\/strong><\/h3>\n\n\n\n

Y. Nasu<\/strong>, C. Murphy-Royal, Y. Wen, J. Haidey, M.R.S. Molina, A. Aggarwal, S. Zhang<\/strong>, Y. Kamijo<\/strong>, M.-E. Paquet, K. Podgorski, M. Drobizhev, J.S. Bains, M.J. Lemieux, G.R. Gordon, R.E. Campbell*, \u201cA genetically encoded fluorescent biosensor for extracellular L-lactate<\/a>\u201d, Nat. Commun.,<\/em> 2021, 12, <\/strong>7058. Preprint posted to bioRxiv<\/em> 2021.03.05.434048<\/a>.<\/p>\n\n\n\n

118. Design and prototyping of genetically encoded arsenic biosensors based on transcriptional regulator AfArsR<\/strong><\/h3>\n\n\n\n

S.S. Khan<\/strong>*, Y. Shen,<\/strong> M.Q. Fatmi, R.E. Campbell, and H. Bokhari*, \u201cDesign and prototyping of genetically encoded arsenic biosensors based on transcriptional regulator AfArsR<\/a>\u201d, Biomolecules<\/em>, 2021, 11<\/strong>, 1276.<\/p>\n\n\n\n

117. Photocleavable proteins that undergo fast and efficient dissociation<\/strong><\/h3>\n\n\n\n

X. Lu<\/strong>, Y. Wen, S. Zhang<\/strong>, W. Zhang<\/strong>, Y. Chen<\/strong>, Y. Shen<\/strong>, M.J. Lemieux, and R.E. Campbell*, \u201cPhotocleavable proteins that undergo fast and efficient dissociation<\/a>\u201d. Chem. Sci.<\/em>, 2021,12<\/strong>, 9658-9672, Advance Article<\/strong>. Posted to bioRxiv<\/em> 2020.12.10.419556<\/a>.
Supplementary Movie 1<\/a>
Supplementary Movie 2<\/a>
Supplementary Movie 3<\/a>
Supplementary information<\/a><\/p>\n\n\n\n

116. Structure- and mechanism-guided design of single fluorescent protein-based biosensors<\/strong><\/h3>\n\n\n\n

Y. Nasu<\/strong>, Y. Shen<\/strong>, L. Kramer<\/strong>, and R.E. Campbell*, \u201cStructure- and mechanism-guided design of single fluorescent protein-based biosensors<\/a>\u201d, Nat. Chem. Biol. <\/em>2021, 17<\/strong>, 509\u2013518. doi:<\/strong> https:\/\/doi.org\/10.1038\/s41589-020-00718-x
PDF version of manuscript<\/a>
Supplementary tables<\/a>
PyMol session file (.pse) with a superposition of all reported FP biosensor crystal structures<\/a> (June 2025 update with additional structures<\/a>)<\/p>\n\n\n\n

115. Controlled Osteogenic Differentiation of Human Mesenchymal Stem Cells Using Dexamethasone-Loaded Light-Responsive Microgels<\/strong><\/h3>\n\n\n\n

Y. Zhang, C. Fang, S. Zhang<\/strong>, R.E. Campbell and M. Serpe*, \u201cControlled Osteogenic Differentiation of Human Mesenchymal Stem Cells Using Dexamethasone-Loaded Light-Responsive Microgels<\/a>\u201d, ACS Appl. Mater. Interfaces, <\/em>2021, 13<\/strong>, 7051\u20137059.<\/p>\n\n\n\n

114. Switching between Ultrafast Pathways Enables a Green-Red Emission Ratiometric Fluorescent-Protein-Based Ca2+<\/sup> Biosensor<\/strong><\/h3>\n\n\n\n

113. Improved genetically encoded near-infrared fluorescent calcium ion indicators for in vivo imaging<\/strong><\/h3>\n\n\n\n

Y. Qian<\/strong>, D.M.O. Cosio, K.D. Piatkevich, S. Aufmkolk, W.-C. Su, O.T. Celiker, A. Schohl, M.H. Murdock, A. Aggarwal<\/strong>, Y.-F. Chang, P.W. Wiseman, E.S. Ruthazer, E.S. Boyden, and R.E. Campbell*, \u201cImproved genetically encoded near-infrared fluorescent calcium ion indicators for in vivo imaging<\/a>\u201d, PLoS Biol<\/em>., 2020, 18<\/strong>, e3000965. Preprint posted to bioRxiv<\/em> 2020.04.08.032433<\/a>.<\/p>\n\n\n\n

112. Engineering photosensory modules of non-opsin-based optogenetic actuators<\/strong><\/h3>\n\n\n\n

X. Lu<\/strong>, Y. Shen<\/strong>, and R.E. Campbell*, \u201cEngineering photosensory modules of non-opsin-based optogenetic actuators<\/a>\u201d, Int. J. Mol. Sci.<\/em>, 2020, 21<\/strong>, 6522.<\/p>\n\n\n\n

111. The role of amino acids in neurotransmission and fluorescent tools for their detection<\/strong><\/h3>\n\n\n\n

R. Dalangin<\/strong>, A. Kim<\/strong>, and R.E. Campbell*, \u201cThe role of amino acids in neurotransmission and fluorescent tools for their detection<\/a>\u201d, Int. J. Mol. Sci.<\/em>, 2020, 21<\/strong>, 6197.<\/p>\n\n\n\n

110. Challenges for therapeutic applications of opsin-based optogenetic tools in humans<\/strong><\/h3>\n\n\n\n

Y. Shen<\/strong>, R.E Campbell, D. C\u00f4t\u00e9 and M.-E. Paquet*, \u201cChallenges for therapeutic applications of opsin-based optogenetic tools in humans<\/a>\u201d, Front. Neural Circuits<\/em>, 2020, 14<\/strong>, 41.<\/p>\n\n\n\n

109. High performance intensiometric direct- and inverse-response genetically encoded biosensors for citrate<\/strong><\/h3>\n\n\n\n

Y. Zhao<\/strong>, Y. Wen*, Y. Shen<\/strong>, and R.E. Campbell*, \u201cHigh performance intensiometric direct- and inverse-response genetically encoded biosensors for citrate<\/a>\u201d, ACS Cent. Sci.<\/em>, 2020, 6<\/strong>, 1441\u20131450. Preprint posted on bioRxiv<\/em> 2020.04.12.038547<\/a>.<\/p>\n\n\n\n

108. A bright and high-performance genetically encoded Ca2+<\/sup> indicator based on mNeonGreen fluorescent protein<\/strong><\/h3>\n\n\n\n

L. Zarowny<\/strong> (equal contribution), A. Aggarwal<\/strong> (equal contribution), V. Rutten, I. Kolb, The GENIE Project, R. Patel, H.-Y. Huang, Y.-F. Chang, T. Phan<\/strong>, R. Kanyo, M. Ahrens, W. T. Allison, K. Podgorski, and R.E. Campbell*, \u201cA bright and high-performance genetically encoded Ca2+<\/sup> indicator based on mNeonGreen fluorescent protein<\/a>\u201d, ACS Sensors<\/em>, 2020, 5, 7<\/strong>, 1959\u20131968. Preprint posted on bioRxiv<\/em> 2020.01.16.909291<\/a>.<\/p>\n\n\n\n

107. Intelligent image-activated cell sorting 2.0<\/strong><\/h3>\n\n\n\n

A. Isozaki, H. Mikami, H. Tezuka, H. Matsumura, K. Huang, M. Akamine, K. Hiramatsu, T. Iino, T. Ito, H. Karakawa, Y. Kasai, Y. Li<\/strong>, Y. Nakagawa, S. Ohnuki, T. Ota, Y. Qian<\/strong>, S. Sakuma, T. Sekiya, Y. Shirasaki, N. Suzuki, E. Tayyabi, T. Wakamiya, M. Xu, M. Yamagishi, H. Yan, Q. Yu, S. Yan, D. Yuan, W. Zhang<\/strong>, Y. Zhao, F. Arai, R.E. Campbell, C. Danelon, D. Di Carlo, K. Hiraki, Y. Hoshino, Y. Hosokawa, M. Inaba, A. Nakagawa, Y. Ohya, M. Oikawa, S. Uemura, Y. Ozeki, T. Sugimura, N. Nitta and K. Goda*, \u201cIntelligent image-activated cell sorting 2.0<\/a>\u201d, Lab Chip<\/em>, 2020, 20<\/strong>, 2263-2273<\/p>\n\n\n\n

106. Engineering genetically encoded fluorescent indicators for imaging of neural activity: progress and prospects<\/strong><\/h3>\n\n\n\n

Y. Shen<\/strong>, Y. Nasu<\/strong>, I. Shkolnikov<\/strong>, A. Kim<\/strong>, and R.E. Campbell, \u201cEngineering genetically encoded fluorescent indicators for imaging of neural activity: progress and prospects<\/a>\u201d, Neurosci. Res.<\/em>, 2020, 152<\/strong>, 3-14.<\/p>\n\n\n\n

105. Microfluidic cell sorter for multiparameter screening in directed evolution<\/strong><\/h3>\n\n\n\n

Y.(Yufeng) Zhao<\/strong>, W. Zhang<\/strong>, Y.(Yongxin) Zhao<\/strong>, R.E. Campbell, and D.J. Harrison*, \u201cMicrofluidic cell sorter for multiparameter screening in directed evolution<\/a>\u201d, Lab Chip<\/em>, 2019, 19<\/strong>, 3880-3887.<\/p>\n\n\n\n

104. Ratiometric Detection of Nerve Agents by Coupling Complementary Properties of Silicon-Based Quantum Dots and Green Fluorescent Protein<\/strong><\/h3>\n\n\n\n

C.J Robidillo, S. Wandelt, R. Dalangin<\/strong>, L. Zhang, H. Yu, A. Meldrum, R.E. Campbell, and J.G.C. Veinot*, \u201cRatiometric Detection of Nerve Agents by Coupling Complementary Properties of Silicon-Based Quantum Dots and Green Fluorescent Protein<\/a>\u201d, ACS Appl. Mater. Interfaces<\/em>, 2019, 11<\/strong>, 33478-33488.<\/p>\n\n\n\n

103. Voltage imaging and optogenetics reveal behaviour-dependent changes in hippocampal dynamics<\/strong><\/h3>\n\n\n\n

Y. Adam, J.J. Kim, S. Lou, Y. Zhao<\/strong>, M.E. Xie, D. Brinks, H. Wu, M.A. Mostajo-Radji, S. Kheifets, V. Parot, S. Chettih, K.J. Williams, B. Gmeiner, S.L. Farhi, L. Madisen, E.K. Buchanan, I. Kinsella, D. Zhou, L. Paninski, C.D. Harvey, H. Zeng, P. Arlotta, R.E. Campbell and A.E. Cohen*, \u201cVoltage imaging and optogenetics reveal behaviour-dependent changes in hippocampal dynamics<\/a>\u201d, Nature<\/em>, 2019, 569<\/strong>, 413\u2013417.<\/p>\n\n\n\n

102. Understanding the Fluorescence Change in Red Genetically Encoded Calcium Ion Indicators<\/strong><\/h3>\n\n\n\n

R. Molina, Y. Qian<\/strong>, J. Wu<\/strong>, Y. Shen<\/strong>, R.E. Campbell, T. Hughes, and M. Drobizhev, \u201cUnderstanding the Fluorescence Change in Red Genetically Encoded Calcium Ion Indicators<\/a>\u201c, Biophys. J.<\/em>, 2019, 116<\/strong>, 1873\u20131886. Preprint posted to bioRxiv<\/em>doi.org\/10.1101\/435891<\/a>.<\/p>\n\n\n\n

101. Wide-area all-optical neurophysiology in acute brain slices<\/strong><\/h3>\n\n\n\n

S.L. Farhi, V. Parot, A. Grama, M. Yamagata, A.S. Abdelfattah<\/strong>, Y. Adam, S. Lou, J.J. Kim, R.E. Campbell, D.D. Cox, and A.E. Cohen*, \u201cWide-area all-optical neurophysiology in acute brain slices<\/a>\u201d, J. Neurosci.<\/em> 2019, 39<\/strong>, 4889\u20134908. Preprint posted to bioRxiv<\/em> doi.org\/10.1101\/433953<\/a>.<\/p>\n\n\n\n

100. A genetically encoded near-infrared fluorescent calcium ion indicator<\/strong><\/h3>\n\n\n\n

Y. Qian <\/strong>(equal contribution), K.D. Piatkevich (equal contribution), B. McLarney (equal contribution), A.S. Abdelfattah, S. Mehta, M.H. Murdock, S. Gottschalk, R.S. Molina, W. Zhang<\/strong>, Y. Chen<\/strong>, J. Wu<\/strong>, M. Drobizhev, T.E. Hughes, J. Zhang, E.R. Schreiter, S. Shoham, D. Razansky, E.S. Boyden, and R.E. Campbell*, \u201cA genetically encoded near-infrared fluorescent calcium ion indicator<\/a>\u201d, Nat. Methods<\/em>, 2019, 16<\/strong>, 171\u2013174.<\/p>\n\n\n\n

99. Genetically encoded fluorescent indicators for imaging intracellular potassium ions<\/strong><\/h3>\n\n\n\n

Y. Shen<\/strong>, S-Y. Wu<\/strong>, V. Rancic, A. Aggarwal<\/strong>, Y. Qian<\/strong>, S.-I. Miyashita, K. Ballanyi, R.E. Campbell*, and M. Dong*, \u201cGenetically encoded fluorescent indicators for imaging intracellular potassium ions<\/a>\u201d, Commun. Biol<\/em>., 2019, 2<\/strong>, 18. Preprint posted to bioRxiv <\/em>doi.org\/10.1101\/254383.<\/p>\n\n\n\n

98. A bioluminescent Ca2+<\/sup> indicator based on a topological variant of GCaMP6s<\/strong><\/h3>\n\n\n\n

Y. Qian<\/strong>, V. Rancic, J. Wu<\/strong>, K. Ballanyi, and R.E. Campbell* , \u201cA bioluminescent Ca2+<\/sup> indicator based on a topological variant of GCaMP6s<\/a>\u201d, ChemBioChem<\/em>, 2019, 20<\/strong>, 516-520.<\/p>\n\n\n\n

\"\"<\/p>\n\n\n\n

97. Optogenetic Reporters for Cell Biology and Neuroscience<\/strong><\/h3>\n\n\n\n

W. Zhang<\/strong> and R.E. Campbell*, \u201cOptogenetic Reporters for Cell Biology and Neuroscience<\/a>\u201d in Optogenetics: Light-driven Actuators and Light-emitting Sensors in Cell Biology<\/em><\/a>, Ed. Sophie Vriz and Takeaki Ozawa. Royal Society of Chemistry, September 2019, pages 63 \u2013 98. [eISBN: 978-1-78801-328-4]<\/p>\n\n\n\n

96. In vivo photoacoustic difference-spectra imaging of bacteria using photoswitchable chromoproteins<\/strong><\/h3>\n\n\n\n

R.K.W. Chee, Y. Li, W. Zhang<\/strong>, R.E. Campbell, and R.J. Zemp*, \u201cIn vivo photoacoustic difference-spectra imaging of bacteria using photoswitchable chromoproteins<\/a>\u201d, J. Biomed. Opt.<\/em>, 2018, 23<\/strong>, 106006.<\/p>\n\n\n\n

95. Monomerization of far-red fluorescent proteins<\/strong><\/h3>\n\n\n\n

T.M. Wannier*, S.K. Gillespie, N. Hutchins, R.S. McIsaac, S-Y. Wu<\/strong>, Y. Shen<\/strong>, R.E. Campbell, K.S. Brown, and S.L. Mayo*, \u201cMonomerization of far-red fluorescent proteins<\/a>\u201d, Proc. Natl. Acad. Sci. U.S.A., <\/em>2018, 115<\/strong>, E11294-E11301.<\/p>\n\n\n\n

94. Unnaturally aglow with a bright inner light<\/strong><\/h3>\n\n\n\n

Y. Nasu<\/strong> and Robert E. Campbell*, \u201cUnnaturally aglow with a bright inner light<\/a>\u201c, Science, <\/em>2018, 359<\/strong>, 868-869. [not peer reviewed]<\/p>\n\n\n\n

93. Inverse-response Ca2+<\/sup> indicators for optogenetic visualization of inhibitory synapse activity<\/strong><\/h3>\n\n\n\n

Y. (Yufeng) Zhao, <\/strong>D. Bushey, Y. (Yongxin) Zhao<\/strong>, E.R. Schreiter, D.J. Harrison, A.M. Wong*, and R.E. Campbell*, \u201cInverse-response Ca<\/a>2+<\/sup><\/a> indicators for optogenetic visualization of inhibitory synapse activity<\/a>\u201d, Sci. Rep., <\/em>2018, 8<\/strong>, 11758.<\/p>\n\n\n\n

92. Enhancing fluorescent protein photostability through robot assisted photobleaching<\/strong><\/h3>\n\n\n\n

M.D. Wiens<\/strong>, F. Hoffmann<\/strong>, Y. Chen<\/strong>, and R.E. Campbell*, \u201cEnhancing fluorescent protein photostability through robot assisted photobleaching<\/a>\u201d, Integr. Biol.<\/em>, 2018, 10<\/strong>, 419-428.<\/p>\n\n\n\n

91. Auto Sequencer: A DNA Sequence Alignment and Assembly Tool<\/strong><\/h3>\n\n\n\n

A. Aggarwal<\/strong>*, L. Zarowny<\/strong>, and R.E. Campbell, \u201cAuto Sequencer: A DNA Sequence Alignment and Assembly Tool<\/a>\u201d, Spectrum<\/em>, 2018, No. 1<\/strong>.<\/p>\n\n\n\n

Download software here<\/a><\/p>\n\n\n\n

90. Genetically Encoded Glutamate Indicators with Altered Color and Topology<\/strong><\/h3>\n\n\n\n

J. Wu<\/strong>, A.S. Abdelfattah<\/strong>, H. Zhou<\/strong>, A. Ruangkittisakul, Y. Qian<\/strong>, K. Ballanyi and R.E. Campbell*, \u201cGenetically Encoded Glutamate Indicators with Altered Color and Topology<\/a>\u201d, ACS Chem. Biol<\/em>., 2018, 13<\/strong>, 1832\u20131837.<\/p>\n\n\n\n

89. A genetically encoded Ca2+<\/sup> indicator based on circularly permutated sea anemone red fluorescent protein eqFP578<\/strong><\/h3>\n\n\n\n

Correction to Figure S1<\/a><\/p>\n\n\n\n

88. Surveying the landscape of optogenetic methods for detection of protein-protein interactions<\/strong><\/h3>\n\n\n\n

M.D. Wiens<\/strong> and R.E. Campbell*, \u201cSurveying the landscape of optogenetic methods for detection of protein-protein interactions<\/a>\u201d, Wiley Interdiscip. Rev. Syst. Biol. Med., <\/em>2018,<\/em> 10<\/strong>, e1415.<\/p>\n\n\n\n

87. Blue-Shifted Green Fluorescent Protein Homologues Are Brighter than Enhanced Green Fluorescent Protein under Two-Photon Excitation<\/strong><\/h3>\n\n\n\n

R.S. Molina, T.M. Tran,<\/strong> R.E. Campbell, G.G. Lambert, A. Salih, N.C. Shaner, T.E. Hughes, and M. Drobizhev*, \u201cBlue-Shifted Green Fluorescent Protein Homologues Are Brighter than Enhanced Green Fluorescent Protein under Two-Photon Excitation<\/a>\u201d, J. Phys. Chem. Lett<\/em>., 2017, 8<\/strong>, 2548\u20132554.<\/p>\n\n\n\n

86. Illuminating Photochemistry of an Excitation Ratiometric Fluorescent Protein Calcium Biosensor<\/strong><\/h3>\n\n\n\n

L. Tang, Y. Wang, W. Liu, Y. Zhao<\/strong>, R.E. Campbell, and C. Fang*, \u201cIlluminating Photochemistry of an Excitation Ratiometric Fluorescent Protein Calcium Biosensor<\/a>\u201d, J. Phys. Chem. B<\/em>, 2017, 121<\/strong>, 3016\u20133023.<\/p>\n\n\n\n

85. Optogenetic Control with a Photocleavable Protein, PhoCl<\/strong><\/h3>\n\n\n\n

W. Zhang <\/strong>(equal contribution), A.W. Lohman (equal contribution), Y. Zhuravlova<\/strong>, X. Lu<\/strong>, M.D. Wiens<\/strong>, H. Hoi<\/strong>, S. Yaganoglu, M.A. Mohr, E.N. Kitova, J.S. Klassen, P. Pantazis, R.J. Thompson, and R.E. Campbell*, \u201cOptogenetic Control with a Photocleavable Protein, PhoCl<\/a>\u201d, Nat. Methods,<\/em> 2017, 14<\/strong>, 391-394.<\/p>\n\n\n\n

84. Engineering of mCherry variants with long Stokes shift, red-shifted fluorescence, and low cytotoxicity<\/strong><\/h3>\n\n\n\n

Y. Shen<\/strong>, Y. Chen<\/strong>, J. Wu<\/strong>, N.C. Shaner, and R.E. Campbell*, \u201cEngineering of mCherry variants with long Stokes shift, red-shifted fluorescence, and low cytotoxicity<\/a>\u201d,PLoS ONE<\/em>, 2017, 12<\/strong>, e0171257.<\/p>\n\n\n\n

83. Distinct intracellular Ca2+<\/sup> dynamics regulate apical constriction and differentially contribute to neural tube closure<\/strong><\/h3>\n\n\n\n

M. Suzuki, M. Sato, H. Koyama, Y. Hara, K. Hayashi, N. Yasue, H. Imamura, T. Fujimori, T. Nagai, R.E. Campbell, and N. Ueno*, \u201cDistinct intracellular Ca2+<\/sup> dynamics regulate apical constriction and differentially contribute to neural tube closure<\/a>\u201d, Development<\/em>, 2017, 144, <\/strong>1307-1316.<\/p>\n\n\n\n

82. Ratiometric and photoconvertible fluorescent protein-based voltage indicator prototypes<\/strong><\/h3>\n\n\n\n

A.S. Abdelfattah<\/strong>, V. Rancic, B. Rawal, K. Ballanyi, and R.E. Campbell*, \u201cRatiometric and photoconvertible fluorescent protein-based voltage indicator prototypes<\/a>\u201d, ChemComm<\/em>, 2016, 52<\/strong>, 14153\u201314156.<\/p>\n\n\n\n

81. A tandem green-red heterodimeric fluorescent protein with high FRET efficiency<\/strong><\/h3>\n\n\n\n

M.D. Wiens<\/strong>, Y. Shen<\/strong>, X. Li, M.A. Salem, N. Smisdom, W. Zhang<\/strong>, A. Brown, and R.E. Campbell*, \u201cA tandem green-red heterodimeric fluorescent protein with high FRET efficiency<\/a>\u201d, ChemBioChem<\/em>, 2016, 17<\/strong>, 2361\u20132367.<\/p>\n\n\n\n

80. The growing and glowing toolbox of fluorescent and photoactive proteins<\/strong><\/h3>\n\n\n\n

E.A. Rodriguez*, R.E. Campbell*, J.Y. Lin*, M.Z. Lin*, A. Miyawaki*, A.E. Palmer*, X. Shu*, J. Zhang* and R.Y. Tsien*, \u201cThe growing and glowing toolbox of fluorescent and photoactive proteins<\/a>\u201d, Trends Biochem. Sci<\/em>. 2017, 42<\/strong>, 111\u2013129.<\/p>\n\n\n\n

79. Roger Y. Tsien (1952 \u2013 2016)<\/strong><\/h3>\n\n\n\n

E.A Rodriguez*, N.C Shaner*, M.Z Lin*, and R.E Campbell*, \u201cRoger Y. Tsien (1952 \u2013 2016)<\/a>\u201d, Nat. Methods <\/em>2016, 13<\/strong>, 893.<\/p>\n\n\n\n

78. Spying on Cells: Toward a Perfect Sleeper Agent<\/strong><\/h3>\n\n\n\n

M.D. Wiens<\/strong>, X. Lu<\/strong>, and R.E. Campbell*, \u201cSpying on Cells: Toward a Perfect Sleeper Agent<\/a>\u201d. Cell Chem. Biol<\/em>. 2016, 23<\/strong>, 756-758.<\/p>\n\n\n\n

77. A bright and fast red fluorescent protein voltage indicator that reports neuronal activity in organotypic brain slices<\/strong><\/h3>\n\n\n\n

A.S. Abdelfattah<\/strong>, S.L. Farhi, Y. Zhao<\/strong>, D. Brinks, P. Zou, A. Ruangkittisakul, J. Platisa, V.A. Pieribone, K. Ballanyi, A.E. Cohen, and R.E. Campbell*, \u201cA bright and fast red fluorescent protein voltage indicator that reports neuronal activity in organotypic brain slices<\/a>\u201d. J. Neurosci.<\/em> 2016, 36<\/strong>, 2458-2472.<\/p>\n\n\n\n

Supplementary material<\/a><\/p>\n\n\n\n

76. Engineering Dark Chromoprotein Reporters for Photoacoustic Microscopy and FRET Imaging<\/strong><\/h3>\n\n\n\n

Y. Li <\/strong>(equal contribution), A. Forbrich (equal contribution), J. Wu<\/strong>, P. Shao, R. E. Campbell* and R. Zemp*, \u201cEngineering Dark Chromoprotein Reporters for Photoacoustic Microscopy and FRET Imaging<\/a>\u201d. Sci. Rep.<\/em>, 2016, 6<\/strong>,  22129.<\/p>\n\n\n\n

75. Pharmacological inhibition of lipid droplet formation enhances the effectiveness of curcumin in glioblastoma<\/strong><\/h3>\n\n\n\n

I. Zhang, Y. Cui, A. Amiri, Y. Ding<\/strong>, R. E. Campbell, and D. Maysinger*, \u201cPharmacological inhibition of lipid droplet formation enhances the effectiveness of curcumin in glioblastoma<\/a>\u201d, Eur. J. Pharm. Biopharm.<\/em>, 2016, 100<\/strong>, 66\u201376.<\/p>\n\n\n\n

74. Altered E. coli membrane protein assembly machinery allows proper membrane assembly of eukaryotic protein vitamin K epoxide reductase<\/strong><\/h3>\n\n\n\n

F. Hatahet, J.L. Blazyk, E. Martineau, E. Mandela, Y. Zhao,<\/strong> R.E. Campbell, J. Beckwith* and D. Boyd, \u201cAltered E. coli <\/em>membrane protein assembly machinery allows proper membrane assembly of eukaryotic protein vitamin K epoxide reductase<\/a>\u201d. Proc. Natl. Acad. Sci. U.S.A. <\/em>2015, 112<\/strong>, 15184\u201315189.<\/p>\n\n\n\n

73. Validating tyrosinase homologue melA as a photoacoustic reporter gene for imaging Escherichia coli<\/strong><\/h3>\n\n\n\n

R.J. Paproski (equal contribution), Y. Li <\/strong>(equal contribution), Q. Barber, J.D. Lewis, R.E. Campbell, and R. Zemp*, \u201cValidating tyrosinase homologue melA as a photoacoustic reporter gene for imaging Escherichia coli<\/em><\/a>\u201d. J. Biomed. Opt. <\/em>2015, 20<\/strong>, 106008.<\/p>\n\n\n\n

72. Fluorescent proteins for neuronal imaging<\/strong><\/h3>\n\n\n\n

Y. Zhao<\/strong> and R.E. Campbell*, \u201cFluorescent proteins for neuronal imaging<\/a>\u201d, in New techniques in systems neuroscience<\/em><\/a>, Ed. A. Douglass. Springer International Publishing, Switzerland, April 2015, pages 57-96. [ISBN: 978-3-319-12912-9]<\/p>\n\n\n\n

71. Red fluorescent proteins (RFPs) and RFP-based biosensors for neuronal imaging applications<\/strong><\/h3>\n\n\n\n

Y. Shen<\/strong>, T. Lai<\/strong>, and R.E. Campbell*, \u201cRed fluorescent proteins (RFPs) and RFP-based biosensors for neuronal imaging applications<\/a>\u201d, Neurophoton.<\/em>, 2015, 2<\/strong>, 031203. [Open Access]<\/p>\n\n\n\n

70. Emerging fluorescent protein technologies<\/strong><\/h3>\n\n\n\n

J.R. Enterina<\/strong>, L. Wu<\/strong>, and R.E. Campbell*, \u201cEmerging fluorescent protein technologies<\/a>\u201d, Curr. Opin. Chem. Biol<\/em>., 2015, 27<\/strong>, 10\u201317.<\/p>\n\n\n\n

69. Unraveling Ultrafast Photoinduced Proton Transfer Dynamics in a Fluorescent Protein Biosensor for Ca2+<\/sup> Imaging<\/strong><\/h3>\n\n\n\n

L. Tang, W. Liu, Y. Wang, Y. Zhao<\/strong>, B.G. Oscar, R.E. Campbell, and C. Fang*, \u201cUnraveling Ultrafast Photoinduced Proton Transfer Dynamics in a Fluorescent Protein Biosensor for Ca2+<\/sup> Imaging<\/a>\u201d, Chem. Eur. J. <\/em>2015, 21<\/strong>, 6481-6490.<\/p>\n\n\n\n

68. Fluorescent biosensors illuminate calcium levels within defined beta-cell endosome subpopulations<\/strong><\/h3>\n\n\n\n

T. Albrecht (equal contribution), Y. Zhao <\/strong>(equal contribution), T.H. Nguyen<\/strong>, R.E. Campbell, and J.D. Johnson*, \u201cFluorescent biosensors illuminate calcium levels within defined beta-cell endosome subpopulations<\/a>\u201d, Cell Calcium<\/em>, 2015, 57<\/strong>, 263-274. [Open access]<\/p>\n\n\n\n

67. Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange<\/strong><\/h3>\n\n\n\n

Y. Ding<\/strong>, J. Li, J.R. Enterina<\/strong>, Y. Shen<\/strong>, I. Zhang, P.H. Tewson, G.C.H. Mo, J. Zhang, A.M. Quinn, T.E. Hughes, D. Maysinger, S.C. Alford<\/strong>, Y. Zhang, and R.E. Campbell*, \u201cRatiometric biosensors based on dimerization-dependent fluorescent protein exchange<\/a>\u201d, Nat. Methods<\/em>, 2015, 12<\/strong>, 195-198.<\/p>\n\n\n\n

Annotated versions of the coding sequence of the constructs listed below in text<\/a> and PDF<\/a>:
Single polypeptide FPX biosensor for calcium ion (Addgene plasmid #60887)<\/a>
Single polypeptide FPX biosensor for caspase-3 (Addgene plasmid #60883)<\/a><\/p>\n\n\n\n

66. A Photochromic and Thermochromic Fluorescent Protein<\/strong><\/h3>\n\n\n\n

Y. Shen<\/strong>, M.D. Wiens<\/strong>, and R.E. Campbell*, \u201cA Photochromic and Thermochromic Fluorescent Protein<\/a>\u201d. RSC Adv<\/em>., 2014, 4<\/strong>, 56762-56765. [Open Access PDF<\/a>]<\/p>\n\n\n\n

65. pHuji, a pH sensitive red fluorescent protein for imaging of exo- and endocytosis<\/strong><\/h3>\n\n\n\n

Y. Shen<\/strong> (equal contribution), M. Rosendale (equal contribution), R.E. Campbell (*correspondance related to new FP variants), and D. Perrais*<\/strong>, \u201cpHuji, a pH sensitive red fluorescent protein for imaging of exo- and endocytosis<\/a>\u201d, J. Cell Biol.<\/em>, 2014, 207<\/strong> (3): 419-432.<\/p>\n\n\n\n

64. A long Stokes shift red fluorescent protein Ca2+<\/sup> indicator for 2-photon and ratiometric imaging<\/strong><\/h3>\n\n\n\n

63. Excited State Structural Events of a Dual-Emission Fluorescent Protein Biosensor for Ca2+<\/sup> Imaging Studied by Femtosecond Stimulated Raman Spectroscopy<\/strong><\/h3>\n\n\n\n

Y. Wang, L. Tang, W. Liu, Y. Zhao<\/strong>, B.G. Oscar, R.E. Campbell, and C. Fang*, \u201cExcited State Structural Events of a Dual-Emission Fluorescent Protein Biosensor for Ca2+<\/sup> Imaging Studied by Femtosecond Stimulated Raman Spectroscopy<\/a>\u201d, J. Phys. Chem. B<\/em>, 2015, 119<\/strong>, 2204-2218.<\/p>\n\n\n\n

62. Red fluorescent genetically encoded Ca2+<\/sup> indicators for use in mitochondria and endoplasmic reticulum<\/strong><\/h3>\n\n\n\n

61. Bright and fast multi-colored voltage reporters via electrochromic FRET<\/strong><\/h3>\n\n\n\n

P. Zou (equal contribution), Y. Zhao<\/strong> (<\/strong>equal contribution), A.D. Douglass, D.R. Hochbaum, D. Brinks, C.A. Werley, D.J. Harrison, R.E. Campbell (*correspondence regarding the library screen), A.E. Cohen*, \u201cBright and fast multicolored voltage reporters via electrochromic FRET<\/a>\u201d, Nat. Commun.<\/em>, 2014, 5<\/strong>, 4625.  [Supplementary Material<\/a>; Funding from NSERC Discovery, CIHR MOP 123514, and graduate scholarships from the University of Alberta and Alberta Innovates to Y.Z.]<\/p>\n\n\n\n

60. Excited state structural dynamics of a dual-emission calmodulin-green fluorescent protein sensor for calcium ion imaging<\/strong><\/h3>\n\n\n\n

B.G. Oscar, W. Liu, Y. Zhao<\/strong>, L. Tang, Y Wang, R.E. Campbell, and C. Fang*, \u201cExcited state structural dynamics of a dual-emission calmodulin-green fluorescent protein sensor for calcium ion imaging<\/a>\u201d, Proc. Natl. Acad. Sci. U.S.A.<\/em>, 2014, 111<\/strong>, 10191\u201310196. [Supplementary Material<\/a>; Funding from NSERC Discovery, CIHR MOP 123514, and graduate scholarships from the University of Alberta and Alberta Innovates to Y.Z.]<\/p>\n\n\n\n

59. All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins<\/strong><\/h3>\n\n\n\n

D.R. Hochbaum (equal contribution), Y. Zhao <\/strong>(equal contribution), S.L. Farhi, N. Klapoetke, C.A. Werley, V. Kapoor, P. Zou, J.M. Kralj, D. Maclaurin, N. Smedemark-Margulies, J. Saulnier, G.L. Boulting, Y. Cho, M. Melkonian, G.K-S. Wong, D.J. Harrison, V.N. Murthy, B. Sabatini, E.S. Boyden (equal contribution), R.E. Campbell (equal contribution; *correspondance related to directed evolution), and A.E. Cohen*, \u201cAll-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins<\/a>\u201d, Nat. Methods<\/em>, 2014, 11<\/strong>, 825\u2013833. [Supplementary Material<\/a>;Sample requests<\/a>; Funding from NSERC Discovery, CIHR MOP 123514, and graduate scholarships from the University of Alberta and Alberta Innovates to Y.Z.; Highlighted by Science Media Centre of Canada<\/a>]<\/p>\n\n\n\n

58.<\/strong> Microfluidic cell sorter-aided directed evolution of a protein-based calcium ion indicator with an inverted fluorescent response<\/strong><\/h3>\n\n\n\n

Y. Zhao<\/strong>, A.S. Abdelfattah<\/strong>, Y. Zhao, A. Ruangkittisakul, K. Ballanyi, R.E. Campbell*, D.J. Harrison*, \u201cMicrofluidic cell sorter-aided directed evolution of a protein-based calcium ion indicator with an inverted fluorescent response<\/a>\u201d, Integr. Biol. (Camb)<\/em>, 2014, 6<\/strong>(7), 714-725. [Open Access PDF<\/a>; Supplementary material<\/a>, Movie 1<\/a>, Movie 2<\/a>; Funding from NSERC Discovery, CIHR MOP 123514, and graduate scholarships from the University of Alberta (Y.Z) and Alberta Innovates to (Y.Z. and A.S.A)]<\/p>\n\n\n\n

57. Engineering and characterizing monomeric fluorescent proteins for live-cell imaging applications<\/strong><\/h3>\n\n\n\n

H-w. Ai<\/strong>, M.A. Baird, Y. Shen<\/strong>, M.W. Davidson*, and R.E. Campbell*, \u201cEngineering and characterizing monomeric fluorescent proteins for live-cell imaging applications<\/a>\u201d. Nat. Protocols<\/em>, 2014, 9<\/strong>, 910-928. [Funding from University of Alberta, CFI, NSERC Discovery grant, and Alberta Ingenuity (Scholarship to Y.S. and a New Faculty Award to R.E.C.)]<\/p>\n\n\n\n

56. Optimization of a Genetically Encoded Biosensor for Cyclin B1-Cyclin Dependent Kinase 1 <\/strong><\/h3>\n\n\n\n

A.S.F. Belal<\/strong>, B.R. Sell, H. Hoi<\/strong>, M.W. Davidson, and R.E. Campbell*, \u201cOptimization of a Genetically Encoded Biosensor for Cyclin B1-Cyclin Dependent Kinase 1<\/a>\u201d. Mol. Biosyst.<\/em>, 2014, 10<\/strong>(2), 191-195. [Open Access PDF<\/a>; Supplementary material<\/a>; Funded by NSERC]<\/p>\n\n\n\n

55. FRET with Fluorescent Proteins<\/strong><\/h3>\n\n\n\n

H. Hoi<\/strong>, Y. Ding<\/strong>, and R.E. Campbell*, \u201cFRET with Fluorescent Proteins<\/a>\u201d, in FRET \u2013 F\u00f6rster Resonance Energy Transfer: From Theory to Applications<\/em>. Eds. Igor Medintz and Niko Hildebrandt. Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany, November 2013, pages 431-473. [Google book preview<\/a>; Funded by NSERC Discovery and CIHR NHG 99085]<\/p>\n\n\n\n

54. An engineered monomeric Zoanthus <\/em>sp. yellow fluorescent protein<\/strong><\/h3>\n\n\n\n

H. Hoi<\/strong>, E.S. Howe, Y. Ding,<\/strong> W. Zhang<\/strong>, M.A. Baird, B.R. Sell, J.R. Allen, M.W. Davidson, and R.E. Campbell*, \u201cAn engineered monomeric <\/a>Zoanthus <\/a><\/em>sp. yellow fluorescent protein<\/a>\u201d, Chem. Biol.<\/em>, 2013, 20<\/strong>, 1296-1304. [Highlighted in the same issue<\/a>; Supplementary Material<\/a>; Funded by NSERC Discovery and Alberta Innovates Technology Futures (AITF) Scholarship to W.Z.]<\/p>\n\n\n\n

53. Mutational analysis of a red fluorescent protein-based calcium ion indicator<\/strong><\/h3>\n\n\n\n

H.J. Carlson<\/strong> and R.E. Campbell*, \u201cMutational analysis of a red fluorescent protein-based calcium ion indicator<\/a>\u201d, Sensors<\/em>, 2013, 13<\/strong>(9), 11507-11521. [Open Access PDF<\/a>; Supplementary Material<\/a>; Funded by NSERC Discovery, NSERC PGSM, and Alberta Ingenuity Scholarship]<\/p>\n\n\n\n

52. Circular permutated red fluorescent proteins and calcium ion indicators based on mCherry<\/strong><\/h3>\n\n\n\n

H.J. Carlson<\/strong> and R.E. Campbell*, \u201cCircular permutated red fluorescent proteins and calcium ion indicators based on mCherry<\/a>\u201d, Protein Eng. Des. Sel., <\/em>2013, 26<\/strong>(12): 763-772. [Supplementary Material<\/a>; Funded by NSERC Discovery, NSERC PGSM, and Alberta Ingenuity Scholarship]<\/p>\n\n\n\n

51. Palmitoylation is the Switch that Assigns Calnexin to Quality Control or ER Calcium Signaling<\/strong><\/h3>\n\n\n\n

E.M. Lynes, A. Raturi, M. Shenkman, C.O. Sandova, M.C. Yap, J. Wu<\/strong>, A. Janowicz, N. Myhill, M.D. Benson, R.E. Campbell, L. G. Berthiaume, G.Z. Lederkremer and T. Simmen*, \u201cPalmitoylation is the Switch that Assigns Calnexin to Quality Control or ER Calcium Signaling<\/a>\u201c, J. Cell Sci., 2013, 126, 3893-3903. [Supplementary Material<\/a>; Funded by CIHR NHG 99085]<\/p>\n\n\n\n

50. Improved orange and red Ca2+<\/sup> indicators and photophysical considerations for optogenetic applications<\/strong><\/h3>\n\n\n\n

J. Wu<\/strong>, L. Liu, T. Matsuda, Y. Zhao<\/strong>, A. Rebane, M. Drobizhev, Y-F. Chang, S. Araki, Y. Arai, K. March, T. E. Hughes, K. Sagou, T. Miyata, T. Nagai*, W-h. Li*, R. E. Campbell*, \u201cImproved orange and red Ca2+<\/sup> indicators and photophysical considerations for optogenetic applications<\/a>\u201d, ACS Chem. Neurosci., <\/em>2013, 4<\/strong>(6), 963-972. [Supplementary Material<\/a>; Funded by CIHR NHG 99085, CIHR MOP 123514, NSERC Discovery, and Alberta Ingenuity Nanotechnology Scholarship to Y.Z.; Highlighted at OpenOptogenetics<\/a>]<\/p>\n\n\n\n

49. Highlightable Ca2+<\/sup> indicators for live cell imaging<\/strong><\/h3>\n\n\n\n

H. Hoi<\/strong>, T. Matsuda, T. Nagai, and R.E. Campbell*, \u201cHighlightable Ca2+<\/sup> indicators for live cell imaging<\/a>\u201d, J. Am. Chem. Soc.<\/em>, 2013, 135<\/strong>(1), 46-49. [Supplementary Material<\/a>; Funded by NSERC Discovery; Highlighted at OpenOptogenetics<\/a>]<\/p>\n\n\n\n

48. Optogenetic Reporters<\/strong><\/h3>\n\n\n\n

S.C. Alford<\/strong>, J. Wu<\/strong>, Y. Zhao<\/strong>, R.E. Campbell, and T. Kn\u00f6pfel*, \u201cOptogenetic Reporters<\/a>\u201d. Biol. Cell.<\/em>, 2013, 105<\/strong>, 14-29. [Funded by CIHR NHG 99085, NSERC Discovery, NSERC CGSD3 to S.C.A., Alberta Ingenuity Ph.D. Scholarship to S.C.A., and Alberta Ingenuity Nanotechnology Scholarship to Y.Z.; Highlighted at ChemistryViews<\/a>]<\/p>\n\n\n\n

47. mMaple: a photoconvertible fluorescent protein for use in multiple imaging modalities<\/strong><\/h3>\n\n\n\n

A.L. McEvoy*, H. Hoi<\/strong>, M. Bates, E. Platonova, P.J. Cranfill, M.A. Baird, M.W. Davidson, H. Ewers, J. Liphardt, and R.E. Campbell*, \u201cmMaple: a photoconvertible fluorescent protein for use in multiple imaging modalities<\/a>\u201d. PLoS ONE<\/em>, 2012, 7<\/strong>(12): e51314. [Open Access PDF<\/a>; Supplementary Material<\/a>; Funded by NSERC Discovery]<\/p>\n\n\n\n

46. Dimerization-Dependent Green and Yellow Fluorescent Proteins<\/strong><\/h3>\n\n\n\n

S.C. Alford,<\/strong> Y. Ding<\/strong>, T. Simmen, and R.E. Campbell*, \u201cDimerization-Dependent Green and Yellow Fluorescent Proteins<\/a>\u201d. ACS Synth. Biol.<\/em>, 2012, 1<\/strong>(12), 569-575. [Cover art<\/a>; Supplementary Material<\/a>;Author Feature<\/a>; Funded by CIHR NHG 99085, NSERC Discovery, NSERC CGSD3 to S.C.A., and Alberta Ingenuity Ph.D. Scholarship to S.C.A]<\/p>\n\n\n\n

45. Portable self-contained cultures for phage and bacteria made of paper and tape<\/strong><\/h3>\n\n\n\n

M. Funes-Huacca,  A. Wu,  E. Szepesvari,  P. Rajendran,  N. Kwan-Wong,  A. Razgulin,  Y. Shen<\/strong>,  J. Kagira,  R.E. Campbell and R. Derda*, \u201cPortable self-contained cultures for phage and bacteria made of paper and tape<\/a>\u201d. Lab Chip<\/em>, 2012, 12<\/strong>, 4269-4278. [Funded by NSERC Discovery and Alberta Ingenuity Nanotechnology Scholarship to Y.S]<\/p>\n\n\n\n

44. Simultaneous detection of Ca2+<\/sup> and diacylglycerol signaling in living cells<\/strong><\/h3>\n\n\n\n

P. Tewson, M. Westenberg, Y. Zhao<\/strong>, R.E. Campbell, A.M. Quinn, T.E. Hughes,* \u201cSimultaneous detection of Ca2+<\/sup> and diacylglycerol signaling in living cells<\/a>\u201d. PLoS ONE<\/em>, 2012, 7<\/strong>(8): e42791. [Open Access PDF<\/a>; Funded by CIHR NHG 99085 and Alberta Ingenuity Nanotechnology Scholarship to Y.Z.]<\/p>\n\n\n\n

43. New Bioanalytical Tools and Devices: Chemistry leads the way<\/strong><\/h3>\n\n\n\n

R.E. Campbell*, \u201cNew Bioanalytical Tools and Devices: Chemistry leads the way<\/a>\u201d. Biotechnology Focus (Bioscienceworld)<\/em>, 2012, 16<\/strong>(4), 7-9. [Highlighting the research of Drs. Gibbs-Davis, Serpe, and Derda; Interactive PDF<\/a>]<\/p>\n\n\n\n

42. Supramolecular hosts that recognize methyllysines and disrupt the interaction between a modified histone tail and its epigenetic reader protein<\/strong><\/h3>\n\n\n\n

K.D. Daze,  T. Pinter,  C.S. Beshara<\/strong>,  A. Ibraheem<\/strong>,  S.A. Minaker,  M.C.F. Ma,  R.J.M. Courtemanche,  R.E. Campbell, and F. Hof*, \u201cSupramolecular hosts that recognize methyllysines and disrupt the interaction between a modified histone tail and its epigenetic reader protein<\/a>\u201d. Chem. Sci<\/em>., 2012, 3<\/strong>, 2695-2699. [Supplementary Material<\/a>; Funded by Alberta Cancer Board and NSERC Discovery]<\/p>\n\n\n\n

41. A Fluorogenic Red Fluorescent Protein Heterodimer<\/strong><\/h3>\n\n\n\n

S.C. Alford<\/strong>, A.S. Abdelfattah<\/strong>, Y. Ding<\/strong>, and R.E. Campbell*, \u201cA Fluorogenic Red Fluorescent Protein Heterodimer<\/a>\u201d. Chem. Biol.<\/em>, 2012, 19<\/strong>, 353-360. [Cover Art<\/a>; Highlighted in Nature Methods<\/a><\/em>; Supplementary Material<\/a>; Funded by CIHR NHG 94487 and 99085, NSERC Discovery, NSERC CGSD3 to S.C.A., and Vanier CGS to A.S.A.]<\/p>\n\n\n\n

\"\"<\/p>\n\n\n\n

40. FRET-based biosensors for multiparameter ratiometric imaging of Ca2+<\/sup> dynamics and caspase-3 activity in single cells<\/strong><\/h3>\n\n\n\n

Y. Ding,<\/strong> H-w. Ai<\/strong>, H. Hoi<\/strong>, R.E. Campbell*, \u201cFRET-based biosensors for multiparameter ratiometric imaging of Ca2+<\/sup> dynamics and caspase-3 activity in single cells<\/a>\u201d. Anal. Chem.<\/em>, 2011, 83<\/strong>, 9687\u20139693. [Supplementary Material<\/a>; Funded by CIHR NHG 94487 and 99085 and NSERC Discovery]<\/p>\n\n\n\n

39. A bacteria colony-based screen for optimal linker combinations in genetically encoded biosensors<\/strong><\/h3>\n\n\n\n

A. Ibraheem<\/strong>, H. Yap<\/strong>, Y. Ding<\/strong>, R.E. Campbell*, \u201cA bacteria colony-based screen for optimal linker combinations in genetically encoded biosensors<\/a>\u201d. BMC Biotechnol.<\/em>, 2011, 11<\/strong>, 105. [Open Access PDF<\/a>; Supplementary Material<\/a>; Funded by Alberta Cancer Board]<\/p>\n\n\n\n

38. An Expanded Palette of Genetically Encoded Ca2+<\/sup> Indicators<\/strong><\/h3>\n\n\n\n

Y. Zhao<\/strong>, S. Araki, J. Wu<\/strong>, T. Teramoto, Y-F. Chang, M. Nakano, A.S. Abdelfattah<\/strong>, M. Fujiwara, T. Ishihara, T. Nagai, and R.E. Campbell*, \u201cAn Expanded Palette of Genetically Encoded Ca2+<\/sup> Indicators<\/a>\u201d, Science<\/em>, 2011, 333<\/strong>, 1888-1891. [Press release<\/a>; Highlighted by C&EN Concentrates<\/a>, Biophotonics<\/a>, and Science Signaling<\/a>; Supplementary Material<\/a>; Funded by CIHR NHG 94487 and 99085, NSERC Discovery, Alberta Ingenuity Nanotechnology Scholarship to Y.Z., and Vanier CGS to A.S.A.]<\/p>\n\n\n\n

37. A Monomeric Photoconvertible Fluorescent Protein for Imaging of Dynamic Protein Localization<\/strong><\/h3>\n\n\n\n

H. Hoi<\/strong>, N.C. Shaner, M.W. Davidson, C.W. Cairo, J. Wang, R.E. Campbell*, \u201cA Monomeric Photoconvertible Fluorescent Protein for Imaging of Dynamic Protein Localization<\/a>\u201d, J. Mol. Biol.<\/em>, 2010, 401<\/strong>, 776-791. [Supplementary Material<\/a>; Funded by NSERC Discovery]<\/p>\n\n\n\n

36. Circularly permuted monomeric red fluorescent proteins with new termini in the \u03b2-sheet<\/strong><\/h3>\n\n\n\n

H.J. Carlson<\/strong>, D. Cotton<\/strong>, and R.E. Campbell*, \u201cCircularly permuted monomeric red fluorescent proteins with new termini in the \u03b2-sheet<\/a>\u201d, Protein Sci.<\/em>, 2010, 19<\/strong>, 1490-1499. [Funded by NSERC Discovery, NSERC USRA to D.C., NSERC PGSM to H.J.C., and Alberta Ingenuity Scholarship to H.J.C.]<\/p>\n\n\n\n

35. Fluorescent reporter proteins<\/strong><\/h3>\n\n\n\n

R.E. Campbell and M.W. Davidson*, \u201cFluorescent reporter proteins<\/a>\u201d, Molecular Imaging with Reporter Genes. Eds. Sanjiv S. Gambhir and Shahriar S. Yaghoubi. Cambridge University Press, New York, NY, July 2010: 3 \u2013 40. [Google book preview<\/a>]<\/p>\n\n\n\n

34. Designs and applications of fluorescent protein-based biosensors<\/strong><\/h3>\n\n\n\n

A. Ibraheem<\/strong> and R.E. Campbell*, \u201cDesigns and applications of fluorescent protein-based biosensors<\/a>\u201d, Curr. Opin. Chem. Biol.<\/em>, 2010, 14<\/strong>, 30-36. [Funded by Alberta Cancer Board]<\/p>\n\n\n\n

33. Molecular Imaging: Editorial Overview<\/strong><\/h3>\n\n\n\n

R.E. Campbell* and C.J. Chang*, \u201cMolecular Imaging: Editorial Overview<\/a>\u201c, Curr. Opin. Chem. Biol.<\/em>, 2010, 14<\/strong>, 1-2. [Co-editor for this Special issue of the journal which had 15 invited reviews.]<\/p>\n\n\n\n

32. Engineered fluorescent proteins: innovations and applications<\/strong><\/h3>\n\n\n\n

M.W. Davidson and R.E. Campbell*, \u201cEngineered fluorescent proteins: innovations and applications<\/a>\u201d, Nat. Methods<\/em>, 2009, 6<\/strong>, 713-717. [Invited Commentary for 5th Anniversary issue.]<\/p>\n\n\n\n

31. Genetically encoded biosensors based on engineered fluorescent proteins<\/strong><\/h3>\n\n\n\n

W.B. Frommer*, M.W. Davidson, R.E. Campbell* \u201cGenetically encoded biosensors based on engineered fluorescent proteins<\/a>\u201d, Chem. Soc. Rev.<\/em>, 2009, 38, 2833-2841. [Supplementary Material<\/a>]<\/p>\n\n\n\n

30. Red fluorescent protein pH biosensor to detect concentrative nucleoside transport<\/strong><\/h3>\n\n\n\n

D.E. Johnson, H-w. Ai<\/strong>, P. Wong<\/strong>, J.D. Young, R.E. Campbell*, and J.R. Casey* \u201cRed fluorescent protein pH biosensor to detect concentrative nucleoside transport<\/a>\u201d, J. Biol. Chem.<\/em>, 2009, 284<\/strong>, 20499-20511. [Supplementary Material<\/a>]<\/p>\n\n\n\n

29. Fluorescent Protein-Based Biosensors: Modulation of Energy Transfer as a Design Principle<\/strong><\/h3>\n\n\n\n

R.E. Campbell*, \u201cFluorescent Protein-Based Biosensors: Modulation of Energy Transfer as a Design Principle<\/a>\u201d, Anal. Chem.,<\/em> 2009, 81<\/strong>(15), 5972\u20135979. [Cover Art<\/a>; Podcast<\/a>]<\/p>\n\n\n\n

28. An engineered tryptophan zipper-type peptide as a molecular recognition scaffold<\/strong><\/h3>\n\n\n\n

Z. Cheng<\/strong> and R.E. Campbell*, \u201cAn engineered tryptophan zipper-type peptide as a molecular recognition scaffold<\/a>\u201d, J. Pept. Sci.<\/em>, 2009, 15<\/strong>, 523-532. [Supplementary Material<\/a>]<\/p>\n\n\n\n

27. Genetically encoded FRET-based biosensors for multiparameter fluorescence imaging<\/strong><\/h3>\n\n\n\n

H.J. Carlson<\/strong>, R.E. Campbell*, \u201cGenetically encoded FRET-based biosensors for multiparameter fluorescence imaging<\/a>\u201d, Curr. Opin. Biotechnol.<\/em>, 2009, 20<\/strong>, 19-27.<\/p>\n\n\n\n

26. Fluorescent proteins<\/strong><\/h3>\n\n\n\n

R.E. Campbell*, \u201cFluorescent proteins<\/a>\u201d, Scholarpedia J.<\/em>, 2008, 3<\/strong>(7), 5410. [Open access; Article accessed more than 95,000 times as of July 2014]<\/p>\n\n\n\n

25. Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors<\/strong><\/h3>\n\n\n\n

H-w. Ai<\/strong>, K.L. Hazelwood, M.W. Davidson, and R.E. Campbell*, \u201cFluorescent protein FRET pairs for ratiometric imaging of dual biosensors<\/a>\u201d, Nat. Methods<\/em>, 2008, 5<\/strong>, 401-403. [Supplementary Material<\/a>; Highlighted in October 2008 issue of Biophotonics<\/a>]<\/p>\n\n\n\n

24. Hue-shifted monomeric variants of Clavularia cyan fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging<\/strong><\/h3>\n\n\n\n

H-w. Ai<\/strong>, S.G. Olenych, P. Wong<\/strong>, M.W. Davidson, and R.E. Campbell*, \u201cHue-shifted monomeric variants of Clavularia cyan fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging<\/a>\u201d, BMC Biol.<\/em>, 2008, 6<\/strong>, 13. [Open Access PDF<\/a>; Designated as a Highly Accessed article]<\/p>\n\n\n\n

23. Teal fluorescent proteins: Characterization of a reversibly photoconvertible variant<\/strong><\/h3>\n\n\n\n

H-w. Ai<\/strong>, and R. E. Campbell*, \u201cTeal fluorescent proteins: Characterization of a reversibly photoconvertible variant<\/a>\u201d, Proc. SPIE<\/em>, 2008, 6868<\/strong>, 68680D.<\/p>\n\n\n\n

22. Computational prediction of absorbance maxima for a structurally diverse series of engineered green fluorescent protein chromophores<\/strong><\/h3>\n\n\n\n

Q.K. Timerghazin, H.J. Carlson<\/strong>, C. Liang, R.E. Campbell,* and A. Brown*, \u201cComputational prediction of absorbance maxima for a structurally diverse series of engineered green fluorescent protein chromophores<\/a>\u201d, J. Phys. Chem B<\/em>, 2008, 112<\/strong>, 2533-2541. [Supplementary Material<\/a>]<\/p>\n\n\n\n

21. Identification of sites within a monomeric red fluorescent protein that tolerate peptide insertion and testing of corresponding circular permutations<\/strong><\/h3>\n\n\n\n

Y. Li<\/strong>, A.M. Sierra<\/strong>, H.-w. Ai<\/strong>, and R.E. Campbell*, \u201cIdentification of sites within a monomeric red fluorescent protein that tolerate peptide insertion and testing of corresponding circular permutations<\/a>\u201d, Photochem. Photobiol.<\/em>, 2008, 84<\/strong>, 111\u2013119. [Supplementary Material<\/a>]<\/p>\n\n\n\n

20. More than just pretty colors: the growing impact of fluorescent proteins in the life science<\/strong><\/h3>\n\n\n\n

H-w. Ai<\/strong> and R.E. Campbell*, \u201cMore than just pretty colors: the growing impact of fluorescent proteins in the life sciences<\/a>\u201d, Biotechnology Focus (Bioscienceworld)<\/em>, 2007, issue 11<\/strong>, 16-18.<\/p>\n\n\n\n

19. In vivo screening identifies a highly folded beta-hairpin peptide with a structured extension<\/strong><\/h3>\n\n\n\n

Z. Cheng<\/strong>, M. Miskolzie, and R.E. Campbell*, \u201cIn vivo screening identifies a highly folded beta-hairpin peptide with a structured extension<\/a>\u201d, ChemBioChem<\/em>, 2007, 8<\/strong>, 880-883. [Cover Art<\/a>; Supplementary Material<\/a>]<\/p>\n\n\n\n

\"\"<\/p>\n\n\n\n

18. Exploration of new chromophore structures leads to the identification of improved blue fluorescent proteins<\/strong><\/h3>\n\n\n\n

H.-w. Ai<\/strong>, N.C. Shaner, Z. Cheng<\/strong>, R.Y. Tsien, and R.E. Campbell*, \u201cExploration of new chromophore structures leads to the identification of improved blue fluorescent proteins<\/a>\u201d, Biochemistry<\/em>, 2007,46<\/strong>, 5904 \u2013 5910. [Featured on the cover of the June 2007 issue of Biophotonics<\/a>]<\/p>\n\n\n\n

17. Structural basis for reversible photobleaching of a green fluorescent protein homologue<\/strong><\/h3>\n\n\n\n

J.N. Henderson, H.-w. Ai<\/strong>, R.E. Campbell, and S.J. Remington*, \u201cStructural basis for reversible photobleaching of a green fluorescent protein homologue<\/a>\u201d, Proc. Natl. Acad. Sci. U.S.A.<\/em>, 2007, 14<\/strong>, 6672-6677. [Supplementary Material<\/a>; Featured in June 2007 issue of Biophotonics<\/a> and April 2007 Science Daily online<\/a>]<\/p>\n\n\n\n

16. Fluorescence-based characterization of genetically encoded peptides that fold in live cells: progress towards a generic hairpin scaffold<\/strong><\/h3>\n\n\n\n

Z. Cheng<\/strong> and R.E. Campbell*, \u201cFluorescence-based characterization of genetically encoded peptides that fold in live cells: progress towards a generic hairpin scaffold\u201d, Proc. SPIE<\/em>, 2007, 6449<\/strong>, 64490S.<\/p>\n\n\n\n

15. Directed evolution of a monomeric, bright, and photostable version of Clavularia<\/em> cyan fluorescent protein: structural characterization and applications in fluorescence imaging<\/strong><\/h3>\n\n\n\n

H-w. Ai<\/strong>, J.N. Henderson, S.J. Remington, and R.E. Campbell*, \u201cDirected evolution of a monomeric, bright, and photostable version of Clavularia<\/em> cyan fluorescent protein: structural characterization and applications in fluorescence imaging<\/a>\u201d, Biochem. J.<\/em>, 2006, 400<\/strong>, 531-540.<\/p>\n\n\n\n

14. Assessing the Structural Stability of Designed \u03b2-Hairpin Peptides in the Cytoplasm of Live Cells <\/strong><\/h3>\n\n\n\n

Z. Cheng<\/strong> and R.E. Campbell*, \u201cAssessing the Structural Stability of Designed \u03b2-Hairpin Peptides in the Cytoplasm of Live Cells<\/a>\u201d, ChemBioChem<\/em>, 2006, 7<\/strong>, 1147-1150.<\/p>\n\n\n\n

13. Realization of \u03b2-lactamase as a versatile fluorogenic reporter<\/strong><\/h3>\n\n\n\n

R.E. Campbell*, \u201cRealization of \u03b2-lactamase as a versatile fluorogenic reporter<\/a>\u201d, Trends Biotech<\/em>., 2004, 22<\/strong>, 208-211.<\/p>\n\n\n\n

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Postdoctoral research at the University of California, San Diego:<\/strong><\/h2>\n\n\n\n

12. Autofluorescent Proteins with Excitation in the Optical Window for Intravital Imaging in Mammals<\/strong><\/h3>\n\n\n\n

M.Z. Lin, M.R. McKeown, H.-L. Ng, T.A. Aguilera, N.C. Shaner, R.E. Campbell, S.R. Adams, L.A. Gross, W. Ma, T. Alber, R.Y. Tsien*, \u201cAutofluorescent Proteins with Excitation in the Optical Window for Intravital Imaging in Mammals<\/a>\u201d, Chem. Biol.<\/em>, 2009, 16<\/strong>, 1169-1179.<\/p>\n\n\n\n

11. Improved monomeric red, orange, and yellow fluorescent proteins derived from Discosoma<\/em> red fluorescent protein<\/strong><\/h3>\n\n\n\n

N.C. Shaner, R.E. Campbell, P.A. Steinbach, B.N.G. Giepmans, A.E. Palmer, and R.Y. Tsien*, \u201cImproved monomeric red, orange, and yellow fluorescent proteins derived from Discosoma<\/em> red fluorescent protein<\/a>\u201d, Nat. Biotechnol.,<\/em> 2004, 22<\/strong>, 1567-1572.<\/p>\n\n\n\n

10. Creating New Fluorescent Probes for Cell Biology<\/strong><\/h3>\n\n\n\n

J. Zhang, R.E. Campbell, A.Y. Ting and R.Y. Tsien*, \u201cCreating New Fluorescent Probes for Cell Biology<\/a>\u201d, Nat. Rev. Mol. Cell Biol<\/em>., 2002, 3<\/strong>, 906-918.<\/p>\n\n\n\n

9. A Monomeric Red Fluorescent Protein<\/strong><\/h3>\n\n\n\n

R.E. Campbell, O. Tour, A.E. Palmer, P.A. Steinbach, G.S. Baird, D.A. Zacharias and R.Y. Tsien*, \u201cA Monomeric Red Fluorescent Protein<\/a>\u201d, Proc. Natl. Acad. Sci. U.S.A.<\/em>, 2002, 99<\/strong>, 7877-7882.<\/p>\n\n\n\n

8. New Biarsenical Ligands and Tetracysteine Motifs for Protein Labeling in Vitro and in Vivo: Synthesis and Biological Applications<\/strong><\/h3>\n\n\n\n

S.R. Adams, R.E. Campbell, L.A. Gross, B.R. Martin, G.K. Walkup, Y. Yao, J. Llopis and R.Y. Tsien*, \u201cNew Biarsenical Ligands and Tetracysteine Motifs for Protein Labeling in Vitro and in Vivo: Synthesis and Biological Applications<\/a>\u201d, J. Am. Chem. Soc.<\/em>, 2002, 124<\/strong>, 6063-6076. <\/p>\n\n\n\n

7. Reducing the Environmental Sensitivity of Yellow Fluorescent Protein: Mechanism and Applications<\/strong><\/h3>\n\n\n\n

O. Griesbeck, G.S. Baird, R.E. Campbell, D.A. Zacharias and R.Y. Tsien*, \u201cReducing the Environmental Sensitivity of Yellow Fluorescent Protein: Mechanism and Applications<\/a>\u201d, J. Biol. Chem<\/em>., 2001, 276<\/strong>, 29188-29194.<\/p>\n\n\n\n

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Graduate research at the University of British Columbia:<\/strong><\/h2>\n\n\n\n

6. The Structure of UDP-N<\/em>-Acetylglucosamine 2-Epimerase Reveals Homology to Phosphoglycosyl Transferases<\/strong><\/h3>\n\n\n\n

R.E. Campbell, S.C. Mosimann, M.E. Tanner*, and N.C.J. Strynadka*, \u201cThe Structure of UDP-N<\/em>-Acetylglucosamine 2-Epimerase Reveals Homology to Phosphoglycosyl Transferases<\/a>\u201d,Biochemistry<\/em>, 2000, 39<\/strong>, 14993-15001.<\/p>\n\n\n\n

5. The First Structure of UDP-Glucose Dehydrogenase Reveals the Catalytic Residues Necessary for the Two-fold Oxidation<\/strong><\/h3>\n\n\n\n

R.E. Campbell, S.C. Mosimann, I. van de Rijn, M. E. Tanner, and N.C.J. Strynadka*, \u201cThe First Structure of UDP-Glucose Dehydrogenase Reveals the Catalytic Residues Necessary for the Two-fold Oxidation<\/a>\u201d, Biochemistry<\/em>, 2000, 39<\/strong>, 7012-7023.<\/p>\n\n\n\n

4. UDP-Glucose Analogues as Inhibitors and Mechanistic Probes of UDP-Glucose Dehydrogenase<\/strong><\/h3>\n\n\n\n

R.E. Campbell and M.E. Tanner*, \u201cUDP-Glucose Analogues as Inhibitors and Mechanistic Probes of UDP-Glucose Dehydrogenase<\/a>\u201d, J. Org. Chem<\/em>., 1999, 64<\/strong>, 9487-9492.<\/p>\n\n\n\n

3. Covalent Adduct Formation with a Mutated Enzyme: Evidence for a Thioester Intermediate in the Reaction Catalyzed by UDP-Glucose Dehydrogenase<\/strong><\/h3>\n\n\n\n

X. Ge, R.E. Campbell, I. van de Rijn, and M.E. Tanner*, \u201cCovalent Adduct Formation with a Mutated Enzyme: Evidence for a Thioester Intermediate in the Reaction Catalyzed by UDP-Glucose Dehydrogenase<\/a>\u201d, J. Am. Chem. Soc<\/em>., 1998, 120<\/strong>, 6613-6614.<\/p>\n\n\n\n

2. Uridine diphospho-alpha-D-gluco-hexodialdose: Synthesis and kinetic competence in the reaction catalyzed by UDP-glucose dehydrogenase<\/strong><\/h3>\n\n\n\n

R.E. Campbell and M.E. Tanner*, \u201cUridine diphospho-alpha-D-gluco-hexodialdose: Synthesis and kinetic competence in the reaction catalyzed by UDP-glucose dehydrogenase\u201d<\/a>, Angew. Chem. Int. Ed. Eng.<\/em> 1997, 36<\/strong>, 1520-1522.<\/p>\n\n\n\n

1. Properties and kinetic analysis of UDP-glucose dehydrogenase from group A streptococci. Irreversible inhibition by UDP-chloroacetol<\/strong><\/h3>\n\n\n\n

R.E. Campbell, R.F. Sala, I. van de Rijn and M.E. Tanner*, \u201cProperties and kinetic analysis of UDP-glucose dehydrogenase from group A streptococci. Irreversible inhibition by UDP-chloroacetol\u201d<\/a>, J. Biol. Chem.,<\/em> 1997, 272<\/strong>, 3416-22.<\/p>\n\n\n\n

<\/p>\n\n\n\n

Note: Author names in bold are members of the Campbell lab who performed the work during their time in the Campbell lab. When the work is a collaboration with a former member who performed the work elsewhere, their name is not bolded. <\/strong><\/h4>\n\n\n\n

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<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Preprint. Ratiometric Fluorescent Protein Biosensors Reveal Citrate Dynamics and Cellular Heterogeneity S. Hario (equal contribution), N. Tamura (equal contribution), B.S. Alladin-Mustan, S. M. Ali, M.S. Macauley, Y. Shen, R.E. Campbell, I. Huppertz*, and K. Takahashi-Yamashiro*, \u201cRatiometric Fluorescent Protein Biosensors Reveal Citrate Dynamics and Cellular Heterogeneity\u201d, bioRxiv, 2026.04.16.718871. Preprint. A StayGold-based calcium ion indicator I. Miyazaki, K.K. Tsao, T. Terai, K. Takahashi-Yamashiro, and R.E. Campbell*, \u201cA StayGold-based calcium ion indicator\u201d, bioRxiv, 2026.03.06.710044. Preprint. Development of a photostable pH biosensor based on mStayGold M. Chang, K. Takahashi-Yamashiro, T. Terai, R.E. Campbell*, Kelvin K. Tsao*, \u201cDevelopment of a photostable pH biosensor based on<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-basic","meta":{"footnotes":""},"class_list":["post-3018","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/campbell.chem.s.u-tokyo.ac.jp\/index.php?rest_route=\/wp\/v2\/pages\/3018","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/campbell.chem.s.u-tokyo.ac.jp\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/campbell.chem.s.u-tokyo.ac.jp\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/campbell.chem.s.u-tokyo.ac.jp\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/campbell.chem.s.u-tokyo.ac.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3018"}],"version-history":[{"count":58,"href":"https:\/\/campbell.chem.s.u-tokyo.ac.jp\/index.php?rest_route=\/wp\/v2\/pages\/3018\/revisions"}],"predecessor-version":[{"id":3357,"href":"https:\/\/campbell.chem.s.u-tokyo.ac.jp\/index.php?rest_route=\/wp\/v2\/pages\/3018\/revisions\/3357"}],"wp:attachment":[{"href":"https:\/\/campbell.chem.s.u-tokyo.ac.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3018"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}

J. Wu<\/strong>, D.L. Prole, Y. Shen<\/strong>, Z. Lin, A. Gnanasekaran, Y. Liu, L. Chen<\/strong>, H. Zhou<\/strong>, S.R.W. Chen, Y.M. Usachev, C.W. Taylor, and R.E. Campbell*, \u201cRed fluorescent genetically encoded Ca2+<\/sup> indicators for use in mitochondria and endoplasmic reticulum<\/a>\u201d, Biochem. J.<\/em>, 2014, 464<\/strong>, 13\u201322. [Supplementary Material<\/a>; Funding from NSERC Discovery, CIHR MOP 123514, and a graduate scholarship from Alberta Innovates to Y.S.]<\/p>\n\n\n\n

J. Wu<\/strong>, A.S. Abdelfattah<\/strong>, L.S. Miraucourt, E. Kutsarova, A. Ruangkittisakul, H. Zhou<\/strong>, K. Ballanyi, G. Wicks, M. Drobizhev, A. Rebane, E.S. Ruthazer, and R.E. Campbell*, \u201cA long Stokes shift red fluorescent protein Ca2+<\/sup> indicator for 2-photon and ratiometric imaging<\/a>\u201d, Nat. Commun<\/em>., 2014, 5<\/strong>, 5262. [Supplementary Material<\/a>; Funding from NSERC Discovery, CIHR MOP 123514, and a Vanier Canada Graduate and Alberta Innovates Health Solutions (AIHS) Scholarships to A.S.A]<\/p>\n\n\n\n

Y. Shen<\/strong>, H. Dana, A.S. Abdelfattah<\/strong>, R. Patel, J. Shea, R.S. Molina, B. Rawal, V. Rancic, Y.-F. Chang, L. Wu<\/strong>, Y. Chen<\/strong>, Y. Qian<\/strong>, M.D. Wiens<\/strong>, N. Hambleton<\/strong>, K. Ballanyi, T.E. Hughes, M. Drobizhev, D.S. Kim, M. Koyama, E.R. Schreiter, and R.E. Campbell*, \u201cA genetically encoded Ca<\/a>2+<\/sup><\/a> indicator based on circularly permutated sea anemone red fluorescent protein eqFP578<\/a>\u201d, BMC Biol<\/em>., 2018, 16<\/strong>, 9. Preprint posted to bioRxiv<\/em> doi.org\/10.1101\/213082<\/a>.<\/em><\/p>\n\n\n\n

L. Tang, S. Zhang<\/strong>, Y. Zhao<\/strong>, N. D. Rozanov, L. Zhu, J. Wu<\/strong>, R.E. Campbell, and C. Fang*, \u201cSwitching between Ultrafast Pathways Enables a Green-Red Emission Ratiometric Fluorescent-Protein-Based Ca2+<\/sup> Biosensor<\/a>\u201d, Int. J. Mol. Sci., <\/em>2021, 22<\/strong>, 445.<\/p>\n\n\n\n